Molecular Identification of Fig Cryptic Virus and Fig Fleck-Associated Virus in Turkey
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Ars Docendi
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Recently, several new viruses infecting fig trees were identified. To assess the presence, distribution and genetic diversity of Fig cryptic virus (FCV) and Fig fleck-associated virus (FFkaV) in fig trees of Turkey, a total of 65 fig samples, which show yellowing, chlorotic, necrotic spots and vein clearing symptoms, were collected from Aegean and Mediterranean regions, which are the most important fig growing regions of Turkey, in spring 2014 and tested by molecular analysis. After cDNA synthesis, FCV and FFkaV specific primer sets of RNA dependent RNA polymerase (RdRp) regions were used for reverse transcription-polymerase chain reaction (RT-PCR) analysis and the PCR products were directly sequenced. The obtained sequences were analyzed and nucleotide sequence analysis confirmed the FCV and FFkaV infections. According to the results, some of the fig trees are infected in Turkey by FCV and FFkaV with an incidence of 20 % and 9.2 %, respectively. BLAST analysis of both FCV and FFkaV has shown high identity with Italian isolates (FCV\ref\NC015494.1 and FFkaV\ref\NC015229.1). For FFkaV, the phylogenetic analysis, constructed from partial RdRp nucleotide sequences, clustered the isolates based on their geographical origin. While the correlation between FFkaV isolates and regions was very high, no correlation between collection regions and FCV isolates was observed. It can be concluded that, fig trees from the most important fig growing regions of Turkey are infected by FCV and FFkaV and it is instrumental to overview of the viral control strategies for fig plantations in Turkey.
Description
Keywords
Ficus carica L., FCV, FFkaV, RT-PCR, RdRp, sequencing
Journal or Series
Romanian Biotechnological Letters
WoS Q Value
Q4
Scopus Q Value
Volume
22
Issue
2
