Molecular Identification of Fig Cryptic Virus and Fig Fleck-Associated Virus in Turkey

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dc.authoridElci, Eminur/0000-0002-6434-6321
dc.contributor.authorElci, Eminur
dc.contributor.authorHancer, Tugce
dc.contributor.authorCaglayan, Kadriye
dc.date.accessioned2024-09-18T20:20:07Z
dc.date.available2024-09-18T20:20:07Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractRecently, several new viruses infecting fig trees were identified. To assess the presence, distribution and genetic diversity of Fig cryptic virus (FCV) and Fig fleck-associated virus (FFkaV) in fig trees of Turkey, a total of 65 fig samples, which show yellowing, chlorotic, necrotic spots and vein clearing symptoms, were collected from Aegean and Mediterranean regions, which are the most important fig growing regions of Turkey, in spring 2014 and tested by molecular analysis. After cDNA synthesis, FCV and FFkaV specific primer sets of RNA dependent RNA polymerase (RdRp) regions were used for reverse transcription-polymerase chain reaction (RT-PCR) analysis and the PCR products were directly sequenced. The obtained sequences were analyzed and nucleotide sequence analysis confirmed the FCV and FFkaV infections. According to the results, some of the fig trees are infected in Turkey by FCV and FFkaV with an incidence of 20 % and 9.2 %, respectively. BLAST analysis of both FCV and FFkaV has shown high identity with Italian isolates (FCV\ref\NC015494.1 and FFkaV\ref\NC015229.1). For FFkaV, the phylogenetic analysis, constructed from partial RdRp nucleotide sequences, clustered the isolates based on their geographical origin. While the correlation between FFkaV isolates and regions was very high, no correlation between collection regions and FCV isolates was observed. It can be concluded that, fig trees from the most important fig growing regions of Turkey are infected by FCV and FFkaV and it is instrumental to overview of the viral control strategies for fig plantations in Turkey.en_US
dc.identifier.endpage12390en_US
dc.identifier.issn1224-5984
dc.identifier.issue2en_US
dc.identifier.startpage12383en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10064
dc.identifier.volume22en_US
dc.identifier.wosWOS:000403059300009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherArs Docendien_US
dc.relation.ispartofRomanian Biotechnological Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFicus carica L.en_US
dc.subjectFCVen_US
dc.subjectFFkaVen_US
dc.subjectRT-PCRen_US
dc.subjectRdRpen_US
dc.subjectsequencingen_US
dc.titleMolecular Identification of Fig Cryptic Virus and Fig Fleck-Associated Virus in Turkeyen_US
dc.typeArticleen_US

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