In vitro culture of in vivo Saanen goat embryos by vitrification

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dc.contributor.authorKöse, Ayşe Merve
dc.contributor.authorTekeli, Tevfik
dc.date.accessioned2019-07-16T16:01:08Z
dc.date.available2019-07-16T16:01:08Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe purpose of this study was to investigate the survival rate of high-quality embryos (n: 101) derived in vivo from Saanen goats (n: 15) in culture following their vitrified freezing and thawing during the breeding season. The in vitro postthaw survival rates of 101 vitrified embryos at 24, 48, and 72 h were 59.4%, 33.6%, and 25.7%, respectively. According to the developmental stage of the embryos, the survival rates were 51.3%, 20.5%, and 15.4% in the morula, and 64.5%, 41.9%, and 32.3% in the blastocyst, respectively. The survival rates of the blastocysts and morulae at 48 and 72 h were significantly different (P < 0.05). While the survival rates of the embryos of Grade 1 quality at 24, 48, and 72 h were 78.6%, 46.4%, and 32.1%, Grade 2 quality survival rates were 35.5%, 17.8%, and 17.8%, respectively. The survival rates of Grades 1 and 2 embryos at 24 h (P < 0.001) and 48 h (P < 0.05) were significantly different. As a result, it was concluded that the embryos to be frozen should be selected according to their development stage and quality, and preferably should be of Grade 1 quality in the blastocyst stage for the cryopreservation of in vivo-derived goat embryos by vitrification.en_US
dc.description.abstractThe purpose of this study was to investigate the survival rate of high-quality embryos (n: 101) derived in vivo from Saanen goats (n: 15) in culture following their vitrified freezing and thawing during the breeding season. The in vitro postthaw survival rates of 101 vitrified embryos at 24, 48, and 72 h were 59.4%, 33.6%, and 25.7%, respectively. According to the developmental stage of the embryos, the survival rates were 51.3%, 20.5%, and 15.4% in the morula, and 64.5%, 41.9%, and 32.3% in the blastocyst, respectively. The survival rates of the blastocysts and morulae at 48 and 72 h were significantly different (P < 0.05). While the survival rates of the embryos of Grade 1 quality at 24, 48, and 72 h were 78.6%, 46.4%, and 32.1%, Grade 2 quality survival rates were 35.5%, 17.8%, and 17.8%, respectively. The survival rates of Grades 1 and 2 embryos at 24 h (P < 0.001) and 48 h (P < 0.05) were significantly different. As a result, it was concluded that the embryos to be frozen should be selected according to their development stage and quality, and preferably should be of Grade 1 quality in the blastocyst stage for the cryopreservation of in vivo-derived goat embryos by vitrification.en_US
dc.identifier.endpage608en_US
dc.identifier.issn1300-0128
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84995931569en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage603en_US
dc.identifier.urihttps://trdizin.gov.tr/publication/paper/detail/TWpFek5EQXhNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12483/2625
dc.identifier.volume40en_US
dc.identifier.wosWOS:000387808500012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Veterinary and Animal Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectZiraaten_US
dc.subjectSütçülük ve Hayvan Bilimlerien_US
dc.titleIn vitro culture of in vivo Saanen goat embryos by vitrificationen_US
dc.typeArticleen_US

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