Characterization of antagonistic and plant growth-promoting traits of endophytic bacteria isolated from bean plants against Pseudomonas syringae pv. phaseolicola
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Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Zirai Mücadele Merkez Araştırma Enstitüsü Müdürlüğü
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Pseudomonas syringae pv. phaseolicola (Psp) is a seed-borne bacterium which causes
halo blight disease in common bean. This study aimed the determination of plant
growth-promoting traits (such as IAA, phosphate solubilization) and antagonistic
potentials (such as siderophore and ammonia productions) of endophytic plant growthpromoting bacteria (PGPB) from healthy bean plants growing in different regions
against Psp in vitro conditions. A total of 131 bacteria were primarily characterized
as putative PGPB and tested for antagonist activity against Psp in dual culture tests.
Seventy-one bacterial isolates demonstrated antagonistic activities against Psp isolate
in varying ratios. Among these 71 isolates, 30 representative bacterial isolates from the
different regions/fields were selected. On the basis of morphological, physiological,
biochemical characteristics and confirmation by MALDI-TOF analyses, 30 endophytic
antagonist isolates were identified as belonging to 10 genera, 24 different species.
According to results obtained, 10 isolates belong to Bacillus spp., 6 isolates belong
to Pseudomonas spp., 4 isolates belong to Rhizobium radiobacter, 2 isolates belong
to Arthrobacter spp., 2 isolates belong to Achromobacter spanius, 2 isolates belong to
Serratia liquefaciens, 1 isolate belongs to Acinetobacter calcoaceticus, Exiguobacterium
sp., Microbacterium hydrocarbonoxydans, and Ochrobactrum anthropi. The largest and
lowest inhibition zone was produced by endophytic bacterial isolates Pseudomonas
gessardii (4.85) and Bacillus licheniformis (1.35). Among the tested antagonist bacterial
isolates, 10 isolates were positive for the production of α-amylase, 7 isolates positive
for phosphate solubilization, 29 isolates positive for siderophore production, 11
isolates positive for protease production. All selected bacterial isolates produced IAA
and ammonia in relatively varying amounts. P. gessardii produced a relatively large
amount of extracellular siderophore (5.83), Exiguobacterium sp. produced a relatively
large amount of extracellular protease (5.25), P. gessardii and O. anthropi produced
a relatively large amount of extracellular IAA (161.39 µg/ml) and Acinetobacter
calcoaceticus produced a relatively large amount of phosphatase (2.63). This is the
first study reporting bean plants harbor endophytes having plant growth promoting
activities with antagonistic potential against Psp
Description
Keywords
Bean, Halo blight, Endophyte, Antagonist, Plant growth promoting bacteria, Biological control
Journal or Series
Bitki Koruma Bülteni
WoS Q Value
Scopus Q Value
Volume
59
Issue
3
Citation
Duman, K., & Soylu, S. (2019). Characterization of antagonistic and plant growth-promoting traits of endophytic bacteria isolated from bean plants against Pseudomonas syringae pv. phaseolicola. Bitki Koruma Bülteni, 59(3), 59-69.
