Investigation of Faecal Carriage of High-Level Gentamicin Resistant Enterococci in Dogs and Cats
[ N/A ]
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Israel Veterinary Medical Assoc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The emergence and spread of high-level gentamicin resistant (HLGR) enterococci have been a concern due to the eliminatation of bactericidal effect between aminoglycosides and cell-wall-active antimicrobials. Therefore, this study to determine the fecal carriage of (HLGR) enterococci in dogs and cats, to investigate the antimicrobial resistance, resistance mechanisms implicated and virulence genes of the isolates. A total of 465 rectal swab samples from dogs (n=226) and cats (n=239) from three different cities (Istanbul, Ankara and Mersin) of Turkey were used for the analysis of HLGR enterococci. The antimicrobial susceptibilities of HLGR isolates were searched by disc diffusion method, resistance and virulence genes by polymerase chain reaction (PCR) and minimum inhibitory concentration (MIC) values for gentamicin were determined by macrodilution method. HLGR enterococci were detected in 25 (11.1%) dogs and in 28 (11.7%) cats. Based on PCR results, 12 were identified as Enterococcus feacalis and 41 as Enterococcus faecium. All of the isolates showed a MIC value of >= 2048 mu g/ml for gentamicin, and except one isolate, the rest of the isolates showed multidrug resistance (MDR) phenotype. None of the isolates displayed vancomycin resistance phenotype. The bifunctional enzyme encoded by aac(6)-Ie-aph(2)-Ia were detected in HLGR isolates as well as other aminoglycoside resistance genes at varying rates. Virulens genes were only detected in 11 (20.8%) E. faecalis isolates with different combinations. However, none of the isolates carried the bly gene. The results showed that both dogs and cats are a potential reservoir for MDR HLGR enterococci which may play an important role in the spread of these nosocomial pathogens.
Açıklama
Anahtar Kelimeler
Cat, Dog, Enterococci, Faecal carriage, High Level Gentamicin Resistance, Virulence genes
Kaynak
Israel Journal of Veterinary Medicine
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
77
Sayı
1
