Investigation of Faecal Carriage of High-Level Gentamicin Resistant Enterococci in Dogs and Cats

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dc.contributor.authorAslantas, O.
dc.date.accessioned2024-09-18T20:08:04Z
dc.date.available2024-09-18T20:08:04Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe emergence and spread of high-level gentamicin resistant (HLGR) enterococci have been a concern due to the eliminatation of bactericidal effect between aminoglycosides and cell-wall-active antimicrobials. Therefore, this study to determine the fecal carriage of (HLGR) enterococci in dogs and cats, to investigate the antimicrobial resistance, resistance mechanisms implicated and virulence genes of the isolates. A total of 465 rectal swab samples from dogs (n=226) and cats (n=239) from three different cities (Istanbul, Ankara and Mersin) of Turkey were used for the analysis of HLGR enterococci. The antimicrobial susceptibilities of HLGR isolates were searched by disc diffusion method, resistance and virulence genes by polymerase chain reaction (PCR) and minimum inhibitory concentration (MIC) values for gentamicin were determined by macrodilution method. HLGR enterococci were detected in 25 (11.1%) dogs and in 28 (11.7%) cats. Based on PCR results, 12 were identified as Enterococcus feacalis and 41 as Enterococcus faecium. All of the isolates showed a MIC value of >= 2048 mu g/ml for gentamicin, and except one isolate, the rest of the isolates showed multidrug resistance (MDR) phenotype. None of the isolates displayed vancomycin resistance phenotype. The bifunctional enzyme encoded by aac(6)-Ie-aph(2)-Ia were detected in HLGR isolates as well as other aminoglycoside resistance genes at varying rates. Virulens genes were only detected in 11 (20.8%) E. faecalis isolates with different combinations. However, none of the isolates carried the bly gene. The results showed that both dogs and cats are a potential reservoir for MDR HLGR enterococci which may play an important role in the spread of these nosocomial pathogens.en_US
dc.identifier.endpage37en_US
dc.identifier.issn0334-9152
dc.identifier.issn2304-8859
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85128381749en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage27en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8605
dc.identifier.volume77en_US
dc.identifier.wosWOS:000784141800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIsrael Veterinary Medical Assocen_US
dc.relation.ispartofIsrael Journal of Veterinary Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaten_US
dc.subjectDogen_US
dc.subjectEnterococcien_US
dc.subjectFaecal carriageen_US
dc.subjectHigh Level Gentamicin Resistanceen_US
dc.subjectVirulence genesen_US
dc.titleInvestigation of Faecal Carriage of High-Level Gentamicin Resistant Enterococci in Dogs and Catsen_US
dc.typeArticleen_US

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