Antimicrobial Resistance and Virulence Genes of Enterococci Isolated from Water Buffalo's Subclinical Mastitis

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dc.contributor.authorÜrer, Ece Koldaş
dc.contributor.authorTek, Erhan
dc.contributor.authorAslantaş, Özkan
dc.contributor.authorYılmaz, Mehmet Ali
dc.contributor.authorErgün, Yaşar
dc.date.accessioned2024-09-19T16:23:34Z
dc.date.available2024-09-19T16:23:34Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study aimed to investigate the antimicrobial resistance and virulence genes of enterococci isolated from water buffalo’s subclinical mastitis cases. The antimicrobial susceptibilities of the isolates were determined by the disc diffusion method. Identification at the species level of enterococci, virulence [aggregation substance (asa1), gelatinase (gelE), cytolysin (cylA), enterococcal surface protein (esp), and hyaluronidase (hyl)] and resistance genes [macrolide (ermA, ermB, mefA/E) and tetracycline (tetK, tetL, tetM, tetO, and tetS)] were investigated by polymerase chain reaction (PCR). Overall, Enterococcus spp. was recovered from 65 of 200 (32.5%) mastitic milk samples, comprising E. faecium (n=26), E. durans (n=22), E. faecalis (n=12), and E. hirae (n=5). Most isolates (56.9%) were susceptible to all tested antibiotics. The rest of the isolates showed various rate of resistance against rifampicin (23.1%), tetracycline (21.5%), quinupristin-dalfopristin (10.8%), ciprofloxacin (7.7%), erythromycin (6.2%), and chloramphenicol (3.1%). Out of 65 enterococci, only 16 (24.6%) were detected to have virulence genes, of which 12 were positive for gelE, seven were positive for esp, two were positive for asa1, and one was positive for hlyA. The gene cylA was not detected in any isolate tested. Resistance to tetracycline was mainly associated with tetM. Two erythromycin-resistant isolates were positive for ermB, and one was positive for mefA/E. This study was the first to report species distribution, antimicrobial susceptibility, and virulence traits of enterococci isolated from subclinical mastitis of water buffaloes in Çorum Province, Türkiye.en_US
dc.identifier.doi10.31196/huvfd.1181398
dc.identifier.endpage215en_US
dc.identifier.issn2146-717X
dc.identifier.issn2146-7188
dc.identifier.issue2en_US
dc.identifier.startpage209en_US
dc.identifier.trdizinid1145222en_US
dc.identifier.urihttps://doi.org/10.31196/huvfd.1181398
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1145222
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15973
dc.identifier.volume11en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofHarran Üniversitesi Veteriner Fakültesi Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectWater buffalo.en_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectEnterococcien_US
dc.subjectSubclinical mastitisen_US
dc.subjectVirulenceen_US
dc.subjectWater bufaloen_US
dc.titleAntimicrobial Resistance and Virulence Genes of Enterococci Isolated from Water Buffalo's Subclinical Mastitisen_US
dc.typeArticleen_US

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