Synergistic activities of the essential oils Hypericum perforatum with methotrexate on human breast cancer cell line MCF-7
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Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Certex, INCDTP-ICPI
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Hypericum perforatum (Hypericaceae) is a perennial plant usually known as “Sarikantaron“ that has been reported to have various important biological activities.In this present study, it was aimed to (I) identify the components (ii) show the synergistic activities of the essential oils Hypericum perforatum with methotrexate on human breast cancer cell line MCF-7. A normal [Madin-Darby Bovine Kidney (MDBK) cell line] and a cancer cell line [Human breast adenocarcinoma cell line (MCF-7)] were used in this study. The cell culture were treated with various concentrations of Hypericum perforatum's oils. The cytotoxic activity of the essential oils of Hypericum perforatum on the cell lines was measured using the MTT method and the results were evaluated as IC50. In this study, the presence of trans-caryophyllene, germacrene-D, ?-pinene, trans-cadina-1,4-diene, ?- Selinene, caryophyllene oxide and ?-Selinene were identified as major constituents of Hypericum perforatum's oils. The essential oils of Hypericum perforatum also exhibited anticancer activities against MCF-7 cells. The IC50 values of the essential oils, MTX and the essentialoilsplus MTX weredetermined as 0.78, 6.25 and 0.195 ?g/ml, respectively, But, the essential oils of Hypericum perforatum was found to be non-cytotoxic for MDBK cells. In conclusion, the essential oils of Hypericum perforatum was chemically characterized and ?-muurolene, ?-cadinene, germacrene B, a-copaene, germacrene D, bicyclogermacrene, and (E)-caryophyllene were found to be major constituents. The essential oils of Hypericum perforatum possess significant in vitro anticancer potential. The essential oils of Hypericum perforatum with MTX found to be significant effective against breast cancer cells. Further studies especially in vivo anticancer properties of Hypericum perforatum should be searched. These compounds found to be very promising compounds in the treatment of cancer therapy. © 2016 Certex, INCDTP-ICPI.
Açıklama
6th International Conference on Advanced Materials and Systems, ICAMS 2016 -- 20 October 2016 through 22 October 2016 -- Bucharest -- 139798
Anahtar Kelimeler
Anticancer, Essential oil, Hypericum perforatum, MCF-7 cell line, Synergistic activity
Kaynak
ICAMS Proceedings of the International Conference on Advanced Materials and Systems
WoS Q Değeri
Scopus Q Değeri
N/A
