PROTECTIVE EFFECTS OF TIROFIBAN HYDROCHLORIDE, A GLYCOPROTEIN IIB/IIIA INHIBITOR, ON LIVER ISCHEMIA/REPERFUSION INJURY IN RATS
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Carbone Editore
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Aims: Both ischemia and reperfusion (IR) can cause damages in cells. There are several studies to eliminate the damage. Tirofiban Hydrochloride (TH) is a common glycoprotein Ilb/IIIa inhibitor and is being used for ischemic disorders for many years. We aimed to determine the therapeutic effects of tirofiban in liver ischemia reperfusion injury model in this animal study. Materials and methods: We induced a 45-minute hepatic ischemia via portal vein, hepatic artery and bile ducts and a 60-minute reperfusion immediately after hepatic ischemia in male albino Wistar rats. One of the groups received intraperitoneal 0.25 mg/ml TH 30 minutes before ischemia and other received intraperitoneal 0.25 mg/ml TH 30 minutes before reperfusion. At the end of the experiment, all animals were decapitated and blood and tissue samples were collected. Results: To evaluate hepatic functions, we assessed serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). In hepatic tissue samples, catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) levels were evaluated. We stained hepatic tissue samples with Hematoxylin and Eosin and explored them with a light microscope. Serum AST, ALT, and LDH levels were increased after IR and decreased significantly in the group in which TH was administered. Tissue CAT, SOD, and GSH levels were decreased in IR groups. In TH groups, antioxidant levels were increased when compared to IR groups. Hepatocellular injury that indicates IR-related damage was decreased substantially after TH administration. Conclusion: According to the results of the study, TH decreased the destructive effects of IR. We suggest that TH treatment may can be used in the treatment of hepatic IR damage.
Açıklama
Anahtar Kelimeler
Liver, ischemia reperfusion, Tirofiban Hydrochloride, antioxidants
Kaynak
Acta Medica Mediterranea
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
Cilt
31
Sayı
1
