Effects of accidental ammonium Sulphate poisoning on antioxidant/oxidant status in lambs
[ N/A ]
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ecole Nationale Veterinaire Toulouse
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, the effects of the ammonium sulphate intoxication on the blood antioxidant /oxidant status were investigated in Sakiz crossbred lambs. For that, circulating blood urea nitrogen (BUN), nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), beta-carotene, retinol and ceruloplasmin concentrations were measured in 6 lambs accidentally poisoned with ammonium sulphate and in 6 healthy control lambs. Oral treatment with 10% glutamic acid (1g/kg), 2.5% acetic acid (2.5 mL/kg) and vitamin A (400 IU/kg) was daily administered to diseased animals for five days. Poisoned lambs exhibited neurological signs (sleepiness, ataxia, tonic and clonic spasms) coupled to a rumen atony and acceleration of heart and respiratory rates compared to healthy controls. Biochemically, the circulating MDA, NO and BUN concentrations were markedly increased and the GSH, beta-carotene and vitamin A concentrations were significantly depressed compared to healthy controls whereas the ceruloplasmin concentrations were not significantly altered. After treatment, clinical and biochemical signs were significantly alleviated but, however 2 lambs died. For them, the histopathological examinations after haematoxylin-eosin staining revealed cell degeneration in liver, lungs and kidney associated to mononuclear cell infiltrates and proliferation of Kiipffer cells. These results clearly showed the occurrence of an oxidative stress induced by ammonium sulphate poisoning leading to cell damage and proved the efficiency of a treatment based on organic acids and retinol supplementation.
Açıklama
Anahtar Kelimeler
Lamb, ammonium sulphate, intoxication, oxidative stress, nitric oxide, antioxidant depletion, organic acid, retinol
Kaynak
Revue De Medecine Veterinaire
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
162
Sayı
7
