Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes
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Tarih
2022
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info:eu-repo/semantics/openAccess
Özet
In this study, the prevalence of PDV, which infects cherry (Prunus avium L.) trees and is among the important viral pathogens in\rAdana, Kahramanmaraş and Osmaniye provinces where cherry is extensively produced in the Eastern Mediterranean region of\rTurkey, was investigated. A total of 173 cherry leaf samples with and without symptoms collected from Adana, Kahramanmaraş\rand Osmaniye provinces were tested by DAS-ELISA and RT-PCR methods. PDV was not detected in all samples tested by the DAS-\rELISA method. As a result of the tests performed with RT-PCR, 874 bp long nucleotide sequences belonging to the coat protein\rgene region of the 15 samples were obtained and found positive. After the sequences were obtained, they were compared with\rthe sequences from Turkey and the sequences of PDV reference isolates registered in GenBank. As a result of BLASTn analysis, it\rwas determined that the Turkish PDV cherry isolates showed similarity between 89.58-99.32% at the nucleotide level with the\rPDV reference sequence. Phylogenetic analysis of the nucleotide sequences of the obtained isolates was performed and their\rrelationship with PDV cherry isolates in the world and Turkey was determined
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Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online)
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Cilt
6
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2
