Eritrositlerde L-sistein amino asit exportuna hücre içi ve dışı redoks durumunun etkisi
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Date
2006
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Hatay Mustafa Kemal Üniversitesi
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info:eu-repo/semantics/openAccess
Abstract
1ÖZET(5ø7526ø7/(5'(/6ø67(ø1$0ø12$6ø7(;325781$+h&5(ødø9(',ù,5('2.6'85808181(7.ø6øGlutatyon, antioksidan savunmada önemli bir rol oynar. Bu nedenle glutatyon,eritrositlerde yüksek miktarlarda bulunur. L-sistein amino asidi, eritrositlerde glutatyonVHQWH]L LoLQ JHUHNPHNWHGLU %X oDOà úPDQà Q DPDFà K FUH LoL YH Gà úà redoks durumunun,eritrositlerde L-sistein HNVSRUWXQD HWNLVLQL LQFHOHPHNWLU dDOà úPDGD HULWURVLWOHUL IDUNOà konsantrasyonlardaki L-sistein DPLQR DVLGLQH PDUX] Eà UDNà OPà ú YH VRQUD GDHULWURVLWOHUGHNL VHUEHVW ±6+ NRQVDQWUDV\RQXQX |Oo OP úW U $\Qà \|QWHPOH PXDPHOHedilen eritrositler, sonraki L-VLVWHLQ oà Nà úà oDOà úPDODUà QGD NXOODQà OPà úWà U $\Qà ]DPDQGDà Và Qà Q EX JLULúoà Nà úD RODQ HWNLVL GH÷HUOHQGLULOPLúWLU øON RODUDN ELU glutatyon tüketicisiolan 1-chloro-2,4-dinitrobenzene ile muamele edilen eritrositlerde, buthionineVXOIR[LPLQHYDUOà ÷à QGDYH\RNOX÷XQGD/VLVWHLQoà Nà úà Qà QQDVà OHWNLOHQGL÷LEHOLUOHQPLúWLUL-VLVWHLQ JLULúL oDOà úPDODUà Pà ]GD SOD]PDGD /VLVWHLQ NRQVDQWUDV\RQX DUWWà ÷à QGDHULWURVLWOHULQ EX DUWà úD FHYDS YHUHUHN /sistein DPLQR DVLGLQL K FUH LoLQH DOGà ÷à J|VWHULOPLúWLU (ULWURVLWOHUGHNL VHUEHVW ±6+ NRQVDQWUDV\RQX P0 /sisteinX\JXODQGà ÷à QGDVDDWWH P0¶D \ NVHOLUNHQEXNRQVDQWUDV\RQ P0 /VLVWHLQ X\JXODQGà ÷à QGD  P0¶ D \ NVHOPLúWLU (ULWURVLWOHUGH VHUEHVW ±6+NRQVDQWUDV\RQODUà P0 /VLVWHLQ X\JXODQGà ÷à QGD GDNLNDGD  P0¶ D\ NVHOLUNHQVDDWWHEXNRQVDQWUDV\RQ P0¶D\ NVHOPLúWLU$\Qà ]DPDQGDL-sistein DPLQR DVLGLQLQ oà Nà úà Qà Q GD ]DPDQ YH NRQVDQWUDV\RQD ED÷Oà ROGX÷XEHOLUOHQPLúWLU < NVHN PLNWDUGD /VLVWHLQOH PXDPHOH HGLOPLú HULWURVLWOHUGH \ NVHNmiktarda L-VLVWHLQoà Nà úà J|U O UP0/sisteinle muamele edilen eritrositlerde, hücreGà úà VHUEHVW±6+NRQVDQWUDV\RQXVDDWWH P0¶D\ NVHOLUNHQP0/sisteinle muamele edilen eritrositlerde bu konsantrasyon 1,014 ± 0,002 mM' a\ NVHOPLúWLU  & Và FDNOà N HULWURVLWOHUH /VLVWHLQ JLULúLQL WHVW HGLOHQ E W Qkonsantrasyonlarda, önemli derecede LQKLEH HWPLúWLU 6à FDNOà N DUWà úà EX JLULú ROD\à Qà \HQLGHQ G ]HOWPHNWHGLU %HQ]HU VRQXoODU oà Nà ú oDOà úPDODUà LOH GH HOGH HGLOPLúWLU6RQXoODUà Pà ] D\Qà ]DPDQGD /VLVWHLQLQ LoHUL YH Gà úDUà transportunun, eritrositinRNVLGDWLI GXUXPXQGDQ HWNLOHQGL÷LQL J|VWHUPLúWLU *OXWDW\RQ W NHWLOGL÷LQGH YH glutatyonVHQWH]L HQJHOOHQGL÷LQGH /VLVWHLQLQ K FUH\H JLULúoà Nà úà |QHPOL GHUHFHGH D]DOPà úWà UEritrositler, plazma UHGRNV GXUXPXQXQ GHYDPà YH K FUH LoL JOXWDW\RQXQ EHOLUOHGL÷L /VLVWHLQoà Nà úRUDQà QGDUROR\QDU2006, 46 sayfa
ABSTRACTTHE EFFECT OF INTRACELLULAR AND EXTRACELLULAR REDOX67$78621/&<67(ø1((;3257,1(5<7+52&<7(6Glutathione has an important role in antioxidant defense. For this reason,glutathione is found at rather high amounts in erythrocytes. L-cysteine is required forglutathione synthesis in erythrocytes. The objective of this study was to invastigate theeffect of intracellular and extracellular redoks status on L-cysteine exports inerythrocytes. In the present study, we exposed the erythrocytes to differentconcentrations of L-cysteine and then measured the intracellular free -SHconcentrations. The erythrocytes treated in the same manner were later utilized for thecysteine efflux studies. The effect of temperature on the influx and the efflux processeswere also evaluated. We also determined the rate of L-cysteine efflux in the presenceand absence of Buthionine Sulfoximine in erythrocytes that are pretreated with 1-chloro-2, 4-dinitrobenzene, a glutathione depleter.Our L-cysteine influx studies demonstrated that erythrocytes can respond toincreases in L-cysteine concentration in the extracellular media and influx L-cysteine ina concentration-dependent manner. Free -SH concentration in erythrocytes treated with1 mM L-cysteine reached to 1,64 ± 0,06 mM in 1 hour whereas this concentrationreached to 4,30 ± 0,01 mM in 10 mM L-cysteine treated erythrocytes. Free -SHconcentration in erythrocytes treated with 10 mM L-cysteine reach to 2,48 ± 0,09 mMin 10 minute whereas this concentration, when treated 10 mM L-cysteine in 1 hour,reach to 4,30 ± 0,01 mM. The L-cysteine efflux is also determined to be time andconcentration-dependent. Erythrocytes that are pretreated with higher L-cysteineconcentrations displayed a higher efflux process. Outside concentration of free -SH in 1mM L-cysteine pretreated erythrocytes, reach to 0,200 ± 0,005 mM in 1hour, whereasthis concentration reach to 1,014 ± 0,002 mM with 10 mM L-cysteine pretreatederythrocytes. The influx process is significantly inhibited +4 ºC at all of theconcentration tested. Increasing of the temperature later recovered the influx process.Similar results were obtained with the efflux studies. Our results also indicate that rateof inward and outward transport of L-cysteine is affected by the oxidative status oferythrocytes. When glutathione is depleted and glutathione synthesis is blocked, L-cysteine uptake and the efflux processes are significantly decreased. Erythrocytes play arole in regulation the plasma redoks status and intracellular level of glutathionedetermines the rate of the L-cysteine efflux.2006, 46 Pages
ABSTRACTTHE EFFECT OF INTRACELLULAR AND EXTRACELLULAR REDOX67$78621/&<67(ø1((;3257,1(5<7+52&<7(6Glutathione has an important role in antioxidant defense. For this reason,glutathione is found at rather high amounts in erythrocytes. L-cysteine is required forglutathione synthesis in erythrocytes. The objective of this study was to invastigate theeffect of intracellular and extracellular redoks status on L-cysteine exports inerythrocytes. In the present study, we exposed the erythrocytes to differentconcentrations of L-cysteine and then measured the intracellular free -SHconcentrations. The erythrocytes treated in the same manner were later utilized for thecysteine efflux studies. The effect of temperature on the influx and the efflux processeswere also evaluated. We also determined the rate of L-cysteine efflux in the presenceand absence of Buthionine Sulfoximine in erythrocytes that are pretreated with 1-chloro-2, 4-dinitrobenzene, a glutathione depleter.Our L-cysteine influx studies demonstrated that erythrocytes can respond toincreases in L-cysteine concentration in the extracellular media and influx L-cysteine ina concentration-dependent manner. Free -SH concentration in erythrocytes treated with1 mM L-cysteine reached to 1,64 ± 0,06 mM in 1 hour whereas this concentrationreached to 4,30 ± 0,01 mM in 10 mM L-cysteine treated erythrocytes. Free -SHconcentration in erythrocytes treated with 10 mM L-cysteine reach to 2,48 ± 0,09 mMin 10 minute whereas this concentration, when treated 10 mM L-cysteine in 1 hour,reach to 4,30 ± 0,01 mM. The L-cysteine efflux is also determined to be time andconcentration-dependent. Erythrocytes that are pretreated with higher L-cysteineconcentrations displayed a higher efflux process. Outside concentration of free -SH in 1mM L-cysteine pretreated erythrocytes, reach to 0,200 ± 0,005 mM in 1hour, whereasthis concentration reach to 1,014 ± 0,002 mM with 10 mM L-cysteine pretreatederythrocytes. The influx process is significantly inhibited +4 ºC at all of theconcentration tested. Increasing of the temperature later recovered the influx process.Similar results were obtained with the efflux studies. Our results also indicate that rateof inward and outward transport of L-cysteine is affected by the oxidative status oferythrocytes. When glutathione is depleted and glutathione synthesis is blocked, L-cysteine uptake and the efflux processes are significantly decreased. Erythrocytes play arole in regulation the plasma redoks status and intracellular level of glutathionedetermines the rate of the L-cysteine efflux.2006, 46 Pages
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Keywords
Biyokimya, Biochemistry ; Biyoloji, Eritrosit, 6LVWHLQoà Nà úà Redoks dengesi., Erythrocytes, Cysteine efflux, Redox homeostasis
