Ratlarda deneysel yara modelinde biyoaktif cam'ın yara iyileşmesi üzerine etkisinin değerlerndirilmesi
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Dosyalar
Tarih
2021
Yazarlar
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, deneysel yara modeli sonrası Biyoaktif camın yara iyileşmesindeki etkinliğinin değerlendirilmesi amaçlandı. Çalışmada, 200-300 g ağırlığında 32 adet Wistar albino erkek rat rastgele 4 eşit gruba ayrıldı. Gruplar; kontrol grubu, sham grubu, biyoaktif cam grubu ve dexpanthenol grubu olarak belirlendi. Kontrol grubu ratlara herhangi bir yara oluşturulmadı. Diğer gruptaki ratların dorsal skapular bölgesinde 2x2 cm boyutunda tam kat deri flebi çıkarılarak yara oluşturuldu ve 0.gün olarak kabul edildi. Sham grubundaki ratların yara bölgesine tedavi uygulanmayarak negatif kontrol grubu olarak kabul edildi. Biyoaktif cam ve dexpanthenol grubundaki ratların yara bölgesine 21 gün boyunca günde bir kere lokal tedavi uygulandı. Yara alanlarını değerlendirmek amacıyla 0., 3., 7., 14 ve 21. günlerde ImageJ2x programıyla alan hesaplamaları yapıldı. Tüm gruptaki ratlar 21.gün sonunda sakrifiye edildi. Tüm ratlarda kan ve doku örnekleri alınarak histopatolojik, biyokimyasal ve genetik olarak değerlendirildi. Histopatolojik değerlendirmede biyoaktif camın inflamatuar hücreleri azalttığı (P<0.05), ülserli alanların neredeyse hiç görülmediği tespit edildi. Genetik analizde yara dokusundan ekspresyonu yapılan sitokinlerden TNF-α'nın diğer gruplara göre anlamlı derecede az olduğu tespit edildi (P<0.05). Ayrıca IL-10*, VEGF** ve TGF- β**'nın ise anlamlı düzeyde yüksek olduğu belirlendi (*P<0.05, **P<0.001). Bu sonuçlar doğrultusunda biyoaktif camın yara dokusunda iyileşmeyi arttırdığı ve yara iyileşme sürecine olumlu katkılarının olduğu sonucuna varıldı.
In this study, it was aimed to evaluate the effectiveness of bioactive glass on wound healing in an experimental wound model. Thirty-two male Wistar albino rats, weighing 200-300 g, included in the study were randomly divided into 4 groups. Groups was determined control group sham group, bioactive glass group and dexpanthenol group. No wound was created in the control group rats. A wound was created by removing a 2x2 cm full-thickness skin flap in the dorsal interscapular region of each rat. Wound formation was accepted as day 0. Wound area calculations were made with the ImageJ2x program on days 0, 3, 7, 14, and 21 in order to evaluate wound areas within a twenty one-day period. Sham, bioactive glass, and dexpanthenol groups were treated for 21 days and all groups were sacrificed at the end of the 21st day. Blood and tissue samples were taken from all rats and evaluated histopathologically, biochemically, and genetically. In the histopathological evaluation, it was determined that bioactive glass reduced inflammatory cells (P<0.05), and ulcerated areas were almost never seen. In the genetic analysis, TNF-α, one of the cytokines expressed in the scar tissue, was found to be significantly less than the other groups (P<0.05). In addition, IL-10*, VEGF** and TGF-β** were found to be significantly higher (*P<0.05, **P<0.001). In line with these results, it was concluded that bioactive glass increased the healing of the scar tissue and contributed positively to the wound healing process.
In this study, it was aimed to evaluate the effectiveness of bioactive glass on wound healing in an experimental wound model. Thirty-two male Wistar albino rats, weighing 200-300 g, included in the study were randomly divided into 4 groups. Groups was determined control group sham group, bioactive glass group and dexpanthenol group. No wound was created in the control group rats. A wound was created by removing a 2x2 cm full-thickness skin flap in the dorsal interscapular region of each rat. Wound formation was accepted as day 0. Wound area calculations were made with the ImageJ2x program on days 0, 3, 7, 14, and 21 in order to evaluate wound areas within a twenty one-day period. Sham, bioactive glass, and dexpanthenol groups were treated for 21 days and all groups were sacrificed at the end of the 21st day. Blood and tissue samples were taken from all rats and evaluated histopathologically, biochemically, and genetically. In the histopathological evaluation, it was determined that bioactive glass reduced inflammatory cells (P<0.05), and ulcerated areas were almost never seen. In the genetic analysis, TNF-α, one of the cytokines expressed in the scar tissue, was found to be significantly less than the other groups (P<0.05). In addition, IL-10*, VEGF** and TGF-β** were found to be significantly higher (*P<0.05, **P<0.001). In line with these results, it was concluded that bioactive glass increased the healing of the scar tissue and contributed positively to the wound healing process.
Açıklama
Anahtar Kelimeler
Veteriner Hekimliği, Veterinary Medicine, Yara, Yara iyileşmesi, Biyoaktif cam, Gen ekspresyonu, Rat, Wound, Wound healing, Bioactive glass, Gene expression, Rat
