Identification of germin isoforms in wheat callus
Yükleniyor...
Dosyalar
Tarih
2004
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Akademiai Kiado Zrt
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Application of auxin causes cells of the wheat embryo to undergo dedifferentiation and form callus tissue. The synthesis of germin isoforms (G, G' and psiG) has been selectively associated with different phases of embryonic development and callus formation. G and G' germin isoforms are found to be associated with germination. psiG is an embryogenesis related germin isoform. It is clearly important to distinguish whether callus induction caused a precocious initiation of embryonic maturation-specific pseudogermin synthesis, or whether it effected a switch in the pattern of protein synthesis; eliciting accumulation of the normally germination-related germin proteins. To distinguish between these alternatives, it is necessary to identify the germin protein isoforms accumulated during callus formation. Following the electrophoretic separation of callus protein extracts, the position of germin isomers were detected by Western-blotting. The immunoreactive polypeptides accumulating in callus tissue were determined to be exclusively germin, rather than psiG.
Açıklama
Anahtar Kelimeler
germin, germination, callus, immunoblotting
Kaynak
Cereal Research Communications
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
32
Sayı
3
