Identification of germin isoforms in wheat callus

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dc.contributor.authorCaliskan, M
dc.contributor.authorTuret-Sayin, M
dc.contributor.authorTuran, C
dc.contributor.authorCuming, AC
dc.date.accessioned2024-09-18T20:25:21Z
dc.date.available2024-09-18T20:25:21Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractApplication of auxin causes cells of the wheat embryo to undergo dedifferentiation and form callus tissue. The synthesis of germin isoforms (G, G' and psiG) has been selectively associated with different phases of embryonic development and callus formation. G and G' germin isoforms are found to be associated with germination. psiG is an embryogenesis related germin isoform. It is clearly important to distinguish whether callus induction caused a precocious initiation of embryonic maturation-specific pseudogermin synthesis, or whether it effected a switch in the pattern of protein synthesis; eliciting accumulation of the normally germination-related germin proteins. To distinguish between these alternatives, it is necessary to identify the germin protein isoforms accumulated during callus formation. Following the electrophoretic separation of callus protein extracts, the position of germin isomers were detected by Western-blotting. The immunoreactive polypeptides accumulating in callus tissue were determined to be exclusively germin, rather than psiG.en_US
dc.identifier.endpage361en_US
dc.identifier.issn0133-3720
dc.identifier.issn1788-9170
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-6344233891en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage355en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10259
dc.identifier.volume32en_US
dc.identifier.wosWOS:000224331800008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAkademiai Kiado Zrten_US
dc.relation.ispartofCereal Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectgerminen_US
dc.subjectgerminationen_US
dc.subjectcallusen_US
dc.subjectimmunoblottingen_US
dc.titleIdentification of germin isoforms in wheat callusen_US
dc.typeArticleen_US

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