Postpartum sütçü ineklerde GnRH ve hCG uygulamalarının gebelik oranı üzerine etkisi
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Dosyalar
Tarih
2021
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Çalışma postpartum sütçü ineklerde, suni tohumlama anında tek başına GnRH ve suni tohumlama anında GnRH ile tohumlama sonrası 5. günde hCG'nin birlikte kullanımının gebelik oranları üzerine etkisinin belirlenmesi amacıyla düzenlendi. Çalışma; yaşları 3-6 arasında değişen, postpartum 70-120 günler arasında olan ve herhangi bir reprodüktif probleme rastlanılmayan 120 baş Holstein ırkı inek üzerinde gerçekleştirildi. İneklerin östrüs göstermeleri için herhangi bir hormonal uygulama yapılmadı. Çalışma süresince östrüs belirtileri gösteren ineklerin ovaryum muayeneleri rektal yoldan 6–8 MHz linear problu real-time ultrason (Falco, Pie Medical, Netherlands) ile yapıldı. Hayvanlar rastgele 3 eşit gruba ayrıldı. I. gruba (n=40) suni tohumlama anında kas içi yoldan bir GnRH analoğu olan buserelin asetat içeren Receptal® (0,004 mg Buserelin/ml, İntervet) 10 µg dozda kas içi yolla uygulandı. II. gruba (n=40) suni tohumlama anında kas içi yoldan 10 μg dozda buserelin asetat ve tohumlama sonrası 5. günde 1500 IU hCG (Pregnyl® 3x1500 IU, MSD) kas içi yoldan uygulandı. III. grup ise kontrol grubu olarak bırakıldı, suni tohumlama anında ve sonrasında herhangi bir uygulama yapılmadı. İneklerin gebelik muayeneleri suni tohumlama sonrası 30. günde rektal yoldan ultrasonografik muayene ile yapıldı. Gebelik oranları grup I, II ve III'de sırasıyla % 80 (32/40), %80 (32/40), %57.5 (23/40) olarak belirlendi. Çalışmada gebe kalma oranı bakımından grup I ve II ile kontrol grubu arasında istatistiki bir fark tespit edildi (p<0.05). Sonuç olarak, postpartum sütçü ineklerde gebelik oranlarının artırılması amacıyla suni tohumlama anında tek başına GnRH ve suni tohumlama anında GnRH ile suni tohumlama sonrası 5. günde hCG'nin birlikte kullanımının ineklerde gebelik oranlarını artırdığı belirlendi. Fakat 5. günde yapılan hCG uygulamasının, yalnızca GnRH uygulamasına kıyasla gebelik oranlarını artırmadığı, bu nedenle ilave hCG uygulamasına gerek olmadığı kanısına varıldı.
The study was carried out to determine the effect of of GnRH alone at the time of artificial insemination and GnRH at the time of artificial insemination and the combined use of hCG on the 5th day after insemination in postpartum dairy cows on pregnancy rates. The study was conducted on 120 Holstein cows which were aging between 3-6 years old, the cows were on 70-120 days postpartum and had no reproductive problems. No hormonal application was carried out for ensuring estrus in cows. The ovarian examinations in cows showing signs of estrus during the study were performed rectally with 6–8 MHz linear probe real-time ultrasound (Falco, Pie Medical, Netherlands). The animals were randomly equal assigned into three groups. A GnRH analogue, Receptal® (0.004 mg Buserelin/ml, Intervet) containing buserelin acetate was administered at the time of insemination at a dose of 10 µg to Group I (n=40) intramuscularly. Group II (n=40) was administered 10 μg buserelin acetate intramuscularly at the time of insemination and 1500 IU hCG (Pregnyl® 3x1500 IU, MSD) intramuscularly on the 5th day after insemination. Group III was assumed as the control group and no administration was made during or after insemination. The pregnancy diagnosis was carried out on 30th days after insemination by transrectal ultrasonography Pregnancy rates were determined as 80% (32/40), 80% (32/40), 57.5% (23/40) in Groups I, II and III, respectively. Statistical difference was found between treatment groups and the control group in terms of pregnancy rates. As a result, it was found that the use of GnRH alone at the time of artificial insemination and combined use of GnRH at the time of artificial insemination and hCG on the 5th day after artificial insemination increase the pregnancy rates in postpartum dairy cows. However, it was concluded that hCG administration on the 5th day did not increase pregnancy rates compared to GnRH administration alone, therefore additional hCG administration was not necessary.
The study was carried out to determine the effect of of GnRH alone at the time of artificial insemination and GnRH at the time of artificial insemination and the combined use of hCG on the 5th day after insemination in postpartum dairy cows on pregnancy rates. The study was conducted on 120 Holstein cows which were aging between 3-6 years old, the cows were on 70-120 days postpartum and had no reproductive problems. No hormonal application was carried out for ensuring estrus in cows. The ovarian examinations in cows showing signs of estrus during the study were performed rectally with 6–8 MHz linear probe real-time ultrasound (Falco, Pie Medical, Netherlands). The animals were randomly equal assigned into three groups. A GnRH analogue, Receptal® (0.004 mg Buserelin/ml, Intervet) containing buserelin acetate was administered at the time of insemination at a dose of 10 µg to Group I (n=40) intramuscularly. Group II (n=40) was administered 10 μg buserelin acetate intramuscularly at the time of insemination and 1500 IU hCG (Pregnyl® 3x1500 IU, MSD) intramuscularly on the 5th day after insemination. Group III was assumed as the control group and no administration was made during or after insemination. The pregnancy diagnosis was carried out on 30th days after insemination by transrectal ultrasonography Pregnancy rates were determined as 80% (32/40), 80% (32/40), 57.5% (23/40) in Groups I, II and III, respectively. Statistical difference was found between treatment groups and the control group in terms of pregnancy rates. As a result, it was found that the use of GnRH alone at the time of artificial insemination and combined use of GnRH at the time of artificial insemination and hCG on the 5th day after artificial insemination increase the pregnancy rates in postpartum dairy cows. However, it was concluded that hCG administration on the 5th day did not increase pregnancy rates compared to GnRH administration alone, therefore additional hCG administration was not necessary.
Açıklama
Anahtar Kelimeler
Veteriner Hekimliği, Veterinary Medicine
