Karaciğer parankim kanaması oluşturulan ratlarda İnula viscosa ve Capsella bursa-pastoris ekstre karışımının hemostatik etkisi
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Dosyalar
Tarih
2021
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Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Giriş ve Amaç: Karaciğer cerrahisinde etkili bir kanama kontrolünün sağlanamaması ve transfüzyona bağlı problemler postoperatif mortalite ve morbiditenin en önemli nedenleridir. Bu amaçla topikal hemostatik ajanları da içeren birçok kanama durdurma yöntemi uygulanmaktadır. Bu çalışmada ratlar üzerinde oluşturulan karaciğer laserasyon modeli üzerinde Hatay'da halk arasında geleneksel olarak kanama durdurucu ve antiseptik olarak kullanılan Inula viscosa ve Capsella bursa pastoris bitkilerinin karışımının kanama durdurucu özelliğinin araştırması amaçlandı. Yöntem: Çalışmada 30 rat, 10'arlı üç gruba ayrıldı ve bütün ratlardan preoperatif hemoglobin ölçümü için kan alındı. Daha sonra ratlara standart karaciğer rezeksiyon modeli uygulandı. Rezeksiyon bölgesine 1. grupta spanç, 2. grupta Ankaferd ve 3.grupta Inula viscosa ve Capsella bursa pastoris bitkilerinin ekstre karışımı üç dakika süreyle uygulandı. Postoperatif 5.gün bütün ratların karaciğer örnekleri inflamasyon ve nekroz yoğunluğu açısından değerlendirildi. Bulgular: Postoperatif Hb değerleri spanç grubunda 11,0±1,1 g/dL, Ankaferd grubunda 11,9±2,0 g/dL, İnula grubunda 14,1±1,2 g/dL olarak saptanmıştır (p<0,001). Yapılan histopatolojik incelemede İnula grubunda diğer gruplara göre daha az nekroz gözlenmiş (p=0,001), spanç ve Ankaferd grupları arasında istatistiksel olarak anlamlı bir fark bulunamamıştır. İnula grubunda diğer gruplara göre daha az inflamasyon izlenirken Ankaferd grubunda diğer gruplara göre daha fazla inflamasyon izlenmiştir (p<0,001). Sonuç: Bu çalışmada oluşturulan rat karaciğer rezeksiyon modelinde İnula viscosa ve Capsella bursa pastoris ekstre karışımının Ankaferd ve spanç grubuna kıyasla daha az hemoglobin düşüşüne neden olduğu ve diğer gruplara kıyasla daha az inflamasyon ve nekroz oluşturduğu bulundu.
Introduction and Aim: Failure to achieve an effective bleeding control and problems related to transfusion in liver surgery are the most important causes of postoperative mortality and morbidity. For this purpose, diverse methods of bleeding control approaches, including topical hemostatic agents are used. In this study, it is aimed to investigate the hemostatic properties of the herb extract mixture of Inula viscosa and Capsella bursa pastoris, which have been traditionally used as antiseptic and hemostatic agents in Hatay region, on rat liver laceration model. Method: In this animal study, 30 rats were divided into three groups of 10 and blood samples were taken from all rats for preoperative hemoglobin measurements. Then, the standard liver resection model was applied on all rats. In the first rat group sponge, in the second rat group Ankaferd and in the third rat group Inula viscosa and Capsella bursa pastoris plant extracts were applied to resection areas for three minutes. On postoperative day 5, liver samples of all rats were evaluated in terms of inflammation and necrosis intensity. Results: Postoperative Hb values were found as 11.0 ± 1.1 g / dL in the sponge group, 11.9 ± 2.0 g / dL in the Ankaferd group, 14.1 ± 1.2 g / dL in the Inula group (p <0.001). In the histopathological examination, less necrosis was observed in the Inula group compared to sponge and Ankaferd groups (p = 0.001). Additionally, no statistically significant necrosis difference was observed between the sponge and Ankaferd groups. While less inflammation was observed in the Inula group compared to the other groups, Ankaferd group had the highest inflammation score (p <0.001). Conclusion: In this rat liver resection model study, it was found that the mixture of Inula viscosa and Capsella bursa pastoris extract caused less hemoglobin decrease compared to the Ankaferd and sponge groups and resulted less inflammation and necrosis.
Introduction and Aim: Failure to achieve an effective bleeding control and problems related to transfusion in liver surgery are the most important causes of postoperative mortality and morbidity. For this purpose, diverse methods of bleeding control approaches, including topical hemostatic agents are used. In this study, it is aimed to investigate the hemostatic properties of the herb extract mixture of Inula viscosa and Capsella bursa pastoris, which have been traditionally used as antiseptic and hemostatic agents in Hatay region, on rat liver laceration model. Method: In this animal study, 30 rats were divided into three groups of 10 and blood samples were taken from all rats for preoperative hemoglobin measurements. Then, the standard liver resection model was applied on all rats. In the first rat group sponge, in the second rat group Ankaferd and in the third rat group Inula viscosa and Capsella bursa pastoris plant extracts were applied to resection areas for three minutes. On postoperative day 5, liver samples of all rats were evaluated in terms of inflammation and necrosis intensity. Results: Postoperative Hb values were found as 11.0 ± 1.1 g / dL in the sponge group, 11.9 ± 2.0 g / dL in the Ankaferd group, 14.1 ± 1.2 g / dL in the Inula group (p <0.001). In the histopathological examination, less necrosis was observed in the Inula group compared to sponge and Ankaferd groups (p = 0.001). Additionally, no statistically significant necrosis difference was observed between the sponge and Ankaferd groups. While less inflammation was observed in the Inula group compared to the other groups, Ankaferd group had the highest inflammation score (p <0.001). Conclusion: In this rat liver resection model study, it was found that the mixture of Inula viscosa and Capsella bursa pastoris extract caused less hemoglobin decrease compared to the Ankaferd and sponge groups and resulted less inflammation and necrosis.
Açıklama
Anahtar Kelimeler
Genel Cerrahi, General Surgery, İnula viscosa, Capsella bursa pastoris, Hemostaz, Karaciğer, Kanama, Inula viscosa, Capsella bursa pastoris, Hemostasis, Liver, Bleeding
