Bazı pamuk çeşitlerinin ISSR markörleri ile karakterizasyonu
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Dosyalar
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, kültürü yapılan 30 pamuk çeşidi (Gossypium hirsutum L.) arasındaki genetik ilişkinin ISSR yöntemi kullanılarak belirlenmesi hedeflenmiştir. 30 pamuk çeşidinde polimorfizmin belirlenmesi amacıyla 24 ISSR primeri 8 pamuk genotipinde test edilmiştir. Primerlerin yalnızca 9 tanesi PCR ürünü oluşturmuş ve sonraki çalışmalar bu primerlerle sürdürülmüştür. Seçilen 9 adet ISSR primeri 30 adet pamuk genotipinde toplam 41 bant oluştururken bu bantlardan ortalama 22.3 tanesinin polimorfik olduğu saptanmış, primer başına polimorfik bant sayısı ortalama 2.5 olarak gerçekleşmiştir. Araştırmada kullanılan tüm primerler pamukta polimorfik bant üretirken, polimorfizm oranı primerlere bağlı olarak % 6 ile % 89 arasında değişim göstermiştir. ISSR primerlerine ilişkin polimorfik bilgi içeriği değerleri 0.19 ile 0.68 aralığında değişim göstermiştir. Genotipler arası ortalama Jaccard benzerlik katsayısı 0.77 olarak bulunurken, UPGMA kümeleme analiz sonucu 30 pamuk çeşidi genetik yakınlık açısından 2 ana kümeye ayrılmıştır.
This study was conducted to determine the genetic diversity in 30 cotton cultivars extensively cultivated in Turkey by using ISSR DNA molecular markers. To investigate the genetic diversity in 30 cotton varieties by ISSR molecular marker, 24 ISSR primers were screened in 8 varieties. 9 of 24 which is produced a PCR product were selected according their polymorphism level. These ISSR primers totally produced 41 bands, and 22.3 were polymorphic. The percentage of polymorphic bands per primer was detected as 2.5. The rate of polymorphism depending on the primers ranged between 6% and 89%. Average polimorphism information content was 0.41, with minimum PIC 0.19 and maximum PIC 0.68. While the Jaccard similarity coefficient between the genotypes was detected as 0.77, 30 cotton varieties were groupped within two main clusters in respect to genetic similarity according to UPGMA analyses.
This study was conducted to determine the genetic diversity in 30 cotton cultivars extensively cultivated in Turkey by using ISSR DNA molecular markers. To investigate the genetic diversity in 30 cotton varieties by ISSR molecular marker, 24 ISSR primers were screened in 8 varieties. 9 of 24 which is produced a PCR product were selected according their polymorphism level. These ISSR primers totally produced 41 bands, and 22.3 were polymorphic. The percentage of polymorphic bands per primer was detected as 2.5. The rate of polymorphism depending on the primers ranged between 6% and 89%. Average polimorphism information content was 0.41, with minimum PIC 0.19 and maximum PIC 0.68. While the Jaccard similarity coefficient between the genotypes was detected as 0.77, 30 cotton varieties were groupped within two main clusters in respect to genetic similarity according to UPGMA analyses.
Açıklama
Anahtar Kelimeler
Ziraat, Agriculture
