The red blood cell membrane proteins in rabbits with experimental ketosis
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Tarih
2005
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, deneysel ketozis oluşturulan tavşanlarda eritrosit zar proteinlerinin sodyum dodesil sülfat/poliakrilamid jel elektroforez tekniği (SDS-PAGE) ile incelenmesi amaçlandı. Hayvanlar, ketozis ve kontrol olmak üzere iki gruba ayrıldı. Deneme 13 gün sürdürüldü. Ketozis grubu hayvanlara 5 gün boyunca yem verilmedi ve sonrasında 8 gün besleme uygulandı. Kontrol grubu hayvanlar standart tavşan yemi ile beslendi. Kan örneklerinden izole edilen eritrosit zar proteinleri % 10 akrilamid monomerin kullanıldığı SDS-PAGE tekniği ile analiz edildi. Poliakrilamid jelde ayrımları gerçekleşen eritrosit zar proteinleri dansitometre ile değerlendirildi. Ketozis grubunda, band 3 ile aktin'in 1. günde ve spektrin'in 5. günde önemli düzeyde arttıkları (P < 0,05); GSPD'nin ise 5. günde önemli seviyede azaldığı (P < 0,01) saptandı. Diğer taraftan denemenin 6. günde ankirin'in kontrole göre anlamlı olarak arttığı (P < 0,05) belirlendi. Bu çalışmadan elde edilen sonuçlar, ketozisin, eritrosit zar proteinlerinden band 3, aktin, spektrin, G3PD ve ankirin'de kantitatif değişikliklere yol açabileceğini göstermektedir.
This study investigated the effects of experimentally induced ketosis on erythrocyte membrane proteins in rabbits by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE). The animals were allocated to 2 groups; ketosis and control. The experiment was lasted 13 days. Food was withheld for 5 days in the ketosis group, and then the animals were fed for 8 days. The control group was fed standard rabbit rations. Erythrocyte membrane proteins were isolated from the blood, and analyzed by SDS-PAGE using 10% (w/v) acrylamide monomer. Erythrocyte membrane proteins separated on the polyacrylamide gel were quantified by densitometry. Band 3 and actin on day 1, and spectrin on day 5 were significantly increased (P < 0.05), while glyceraldehyde-3-phosphate dehydrogenase (G3PD, B.C. 1.2.1.12) was significantly decreased (P < 0.01) on day 5 in the ketosis group. On the other hand, ankyrin increased (P < 0.05) when compared to the controls on day 6 of the trial. The results show that ketosis may lead to quantitative changes in erythrocyte membrane proteins including band 3, actin, spectrin, G3PD and ankyrin.
This study investigated the effects of experimentally induced ketosis on erythrocyte membrane proteins in rabbits by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE). The animals were allocated to 2 groups; ketosis and control. The experiment was lasted 13 days. Food was withheld for 5 days in the ketosis group, and then the animals were fed for 8 days. The control group was fed standard rabbit rations. Erythrocyte membrane proteins were isolated from the blood, and analyzed by SDS-PAGE using 10% (w/v) acrylamide monomer. Erythrocyte membrane proteins separated on the polyacrylamide gel were quantified by densitometry. Band 3 and actin on day 1, and spectrin on day 5 were significantly increased (P < 0.05), while glyceraldehyde-3-phosphate dehydrogenase (G3PD, B.C. 1.2.1.12) was significantly decreased (P < 0.01) on day 5 in the ketosis group. On the other hand, ankyrin increased (P < 0.05) when compared to the controls on day 6 of the trial. The results show that ketosis may lead to quantitative changes in erythrocyte membrane proteins including band 3, actin, spectrin, G3PD and ankyrin.
Açıklama
Anahtar Kelimeler
Ziraat, Sütçülük ve Hayvan Bilimleri
Kaynak
Turkish Journal of Veterinary and Animal Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
29
Sayı
1
