Optimization of immobilization conditions of Thermomyces lanuginosus lipase on olive pomace powder using response surface methodology
Yükleniyor...
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this work, microbial lipase from Thermomyces lanuginosus was immobilized onto olive pomace. Response surface methodology was used to optimize the conditions for the maximum activity and to understand the significance and interaction of the factors affecting the specific activity. 5-level-3-factor central composite design was employed to evaluate the effects of immobilization parameters such as enzyme concentration (3-15%, v/v), pH (5.0-9.0) and buffer concentration (20-100 mM) on the specific activity of immobilized lipase. Quadratic polynomial equation was obtained for predicting specific activity of immobilized lipase. The predicted specific activity was 6.00 mu mol p-NP/mg enzyme min under the optimal conditions. Verification experiment (6.21 mu mol p-NP/mg enzyme min) confirmed the validity of the predicted model. Immobilized lipase was used to determine operational stability and it indicated that immobilized lipase was stable retaining more than 80% residual activity after being used repeatedly for 10 consecutive batches of pomace oil transesterification. (C) 2011 Elsevier Ltd. All right sreserved.
Açıklama
Anahtar Kelimeler
Thermomyces lanuginosus, Immobilization, Olive pomace, Optimization, Response surface methodology
Kaynak
Biocatalysis and Agricultural Biotechnology
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
1
Sayı
1
