Optimization of immobilization conditions of Thermomyces lanuginosus lipase on olive pomace powder using response surface methodology

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dc.authoridYucel, Yasin/0000-0002-8572-4213
dc.contributor.authorYucel, Yasin
dc.date.accessioned2024-09-18T20:59:23Z
dc.date.available2024-09-18T20:59:23Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this work, microbial lipase from Thermomyces lanuginosus was immobilized onto olive pomace. Response surface methodology was used to optimize the conditions for the maximum activity and to understand the significance and interaction of the factors affecting the specific activity. 5-level-3-factor central composite design was employed to evaluate the effects of immobilization parameters such as enzyme concentration (3-15%, v/v), pH (5.0-9.0) and buffer concentration (20-100 mM) on the specific activity of immobilized lipase. Quadratic polynomial equation was obtained for predicting specific activity of immobilized lipase. The predicted specific activity was 6.00 mu mol p-NP/mg enzyme min under the optimal conditions. Verification experiment (6.21 mu mol p-NP/mg enzyme min) confirmed the validity of the predicted model. Immobilized lipase was used to determine operational stability and it indicated that immobilized lipase was stable retaining more than 80% residual activity after being used repeatedly for 10 consecutive batches of pomace oil transesterification. (C) 2011 Elsevier Ltd. All right sreserved.en_US
dc.description.sponsorshipMustafa Kemal University Research Foundation [1001M0114]en_US
dc.description.sponsorshipThe author thanks Mustafa Kemal University Research Foundation for providing financial support for this project (Project no. 1001M0114).en_US
dc.identifier.doi10.1016/j.bcab.2011.08.009
dc.identifier.endpage44en_US
dc.identifier.issn1878-8181
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84856900387en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage39en_US
dc.identifier.urihttps://doi.org/10.1016/j.bcab.2011.08.009
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12546
dc.identifier.volume1en_US
dc.identifier.wosWOS:000219122300008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofBiocatalysis and Agricultural Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectThermomyces lanuginosusen_US
dc.subjectImmobilizationen_US
dc.subjectOlive pomaceen_US
dc.subjectOptimizationen_US
dc.subjectResponse surface methodologyen_US
dc.titleOptimization of immobilization conditions of Thermomyces lanuginosus lipase on olive pomace powder using response surface methodologyen_US
dc.typeArticleen_US

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