Subklinik mastitisli inek sütlerinden izole edilen stafilokoklarda antibiyotik duyarlılığı ve plazmit varlığının araştırılması
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Tarih
2010
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info:eu-repo/semantics/openAccess
Özet
Çalışmanın amacı, subklinik mastitisli inek sütlerinden izole edilen Stafilokok izolatlarının bazı antibiyotiklere duyarlılığının araştırılması ve izolatlarda plazmit varlığının belirlenmesidir. Bu amaçla, toplam 51 Stafilokok izolatı incelendi. İzolatlar subklinik mastitisli ineklerden alınan 173 süt örneğinden izole edildi. Bu izolatlardan 47 (%92.15)'si koagulaz negatif, 4 (%7.84)'ü ise koagulaz pozitif olarak değerlendirildi. İzolatlar, mastitiste sıklıkla kullanılan 6 farklı antibiyotiğe duyarlı bulundu. İzole edilen 51 adet Stafilokok izolatından 3 (%5.88)'ünün oksitetrasikline, 3 (%5.88)'ünün sulfametoksazol-trimetoprim kombinasyonuna, 6 (%11.76)'sının novobiyosin'e, 11 (% 21.56)'inin eritromisin'e, 24 (%47.05)'ünün penisilin-G'ye dirençli; 27 (%52.94)'sinin penislin-G'ye, 40 (%78.43)'ının eritromisin'e, 45 (%88.23)'inin novobiyosin'e, 48 (%94.11)'inin oksitetrasikline, 48 (%94.11)'inin sulfametoksazol-trimetoprim kombinasyonuna, 51 (%100.00)'inin amoksisilinklavulanik asit kombinasyonuna duyarlı olduğu tespit edildi. İzolatların 16 (%31.37) tanesinde plazmit tespit edildi. Plazmit taşıyan izolatın 1 (%6.25)'i eritromisine, 2 (%12.50)'si oksitetrasikline, 4 (%25.00)'ü penisilin-G'ye dirençli idi. İzolatların 12 (%75.00)'si penisilin-G'ye, 14 (%87.50)'ü oksitetrasikline, 15 (%93.75)'i eritromisin'e ve tamamının (%100) novobiyosin, amoksisilinklavulanik asit ve sulfametoksazol-trimetoprim kombinasyonlarına duyarlı bulundu. Sonuç olarak koagulaz negatif suşların 16 (%31.37)'sında plazmit tespit edilmesine rağmen, koagulaz pozitif suşlarda plazmit tespit edilmedi.
The purpose of this study was to investigate the susceptibility of Staphylococci isolates from cow milk with subclinical mastitis to some antibiotics and to determine the presence of plasmids in isolates. For this purpose, a total of 51 Staphylococci isolates were examined. These isolates were isolated from 173 milk samples of dairy cows with subclinical mastitis. It was found that 47 (92.15%) isolates were coagulase-negative and 4 (7.84%) were coagulase-positive. These isolates were found to be susceptible to 6 different antibiotics frequently used in mastitis. Three (5.88%) of the 51 Staphylococci isolates were resistant to oxytetracycline, 3 (5.88%) to sulphamethoxazole-trimethoprim, 6 (11.76%) to novobiocin, 11 (21.56%) to erythromycin, and 24 (47.05%) to penicillin-G. Twenty seven (52.94%) isolates were susceptible to penicillin-G, 40 (78.43%) to erythromycin, 45 (88.23%) to novobiocin, 48 (94.11%) to oxytetracycline, 48 (94.11%) to sulphamethoxazole-trimethoprim, and 51 (100%) to amoxycillin-clavulanic acid. Plasmids were detected in 16 (31.37%) of the isolates. One (6.25%) isolate with plasmid was resistant to erythromycin, 2 (12.50%) to oxytetracycline and 4 (25.00%) to penicillin-G. Twelve (75.00%) of them were found to be susceptible to penicillin-G, 14 (87.50%) to oxytetracycline, 15 (93.75%) to erythromycin, and all of them (100.00%) to novobiocin, amoxicillinclavulanic acid and sulphamethoxazole-trimethoprim. In conclusion, although plasmids were found in 16 (31.37%) of the coagulase-negative isolates, there were no plasmids in coagulase-positive isolates.
The purpose of this study was to investigate the susceptibility of Staphylococci isolates from cow milk with subclinical mastitis to some antibiotics and to determine the presence of plasmids in isolates. For this purpose, a total of 51 Staphylococci isolates were examined. These isolates were isolated from 173 milk samples of dairy cows with subclinical mastitis. It was found that 47 (92.15%) isolates were coagulase-negative and 4 (7.84%) were coagulase-positive. These isolates were found to be susceptible to 6 different antibiotics frequently used in mastitis. Three (5.88%) of the 51 Staphylococci isolates were resistant to oxytetracycline, 3 (5.88%) to sulphamethoxazole-trimethoprim, 6 (11.76%) to novobiocin, 11 (21.56%) to erythromycin, and 24 (47.05%) to penicillin-G. Twenty seven (52.94%) isolates were susceptible to penicillin-G, 40 (78.43%) to erythromycin, 45 (88.23%) to novobiocin, 48 (94.11%) to oxytetracycline, 48 (94.11%) to sulphamethoxazole-trimethoprim, and 51 (100%) to amoxycillin-clavulanic acid. Plasmids were detected in 16 (31.37%) of the isolates. One (6.25%) isolate with plasmid was resistant to erythromycin, 2 (12.50%) to oxytetracycline and 4 (25.00%) to penicillin-G. Twelve (75.00%) of them were found to be susceptible to penicillin-G, 14 (87.50%) to oxytetracycline, 15 (93.75%) to erythromycin, and all of them (100.00%) to novobiocin, amoxicillinclavulanic acid and sulphamethoxazole-trimethoprim. In conclusion, although plasmids were found in 16 (31.37%) of the coagulase-negative isolates, there were no plasmids in coagulase-positive isolates.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
21
Sayı
3
