Investigation of antioxidant effect of silibinin molecule on U-2 OS cells induced by hydrogen peroxide

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dc.contributor.authorGündüz, Kübra
dc.contributor.authorEcevit, Hasret
dc.contributor.authorKüçük, Meral Urhan
dc.contributor.authorDırıcan, Emre
dc.date.accessioned2024-09-19T16:26:48Z
dc.date.available2024-09-19T16:26:48Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAim: Silibinin is one of the active constituent of silymarin, an extract of the Silybum\rmarianum L. seeds. There are previous studies that have reported the antioxidant, antiinflammatory, anti-apoptotic and anti-carcinogenic effects of silibinin. However, the antioxidant effect of silibinin on bone cancer have been not evaluated before. In our study,\rwe aimed to evaluate the effect of silibinin on U-2 OS cell proliferation and oxidative stress\rparameters catalase enzyme activity and MDA level.\rMaterials and Methods: U-2 OS cells were exposed to H2O2 (0-800 µM H2O2, for\r24 and 48 hours) to form an oxidative damaged cell model. Moreover, the cells were exposed to silibinin (0-100 µM) for 24 hours. Malondialdehyde (MDA) level and antioxidant\renzyme catalase (CAT) activity were determined by spectrophotometric analysis.\rResults: CAT activity and MDA level were observed to be higher in both H2O2 exposure\rgroups compared to control group (p<0.05). Moreover, CAT activity and MDA level were\robserved to be lower in 500 µM H2O2+silibinin groups in comparation with 500 µM H2O2\rgroup (p<0.05). Similarly, MDA level was determined to be lower in 650 µM H2O2+10\rµM silibinin group in comparation to 650 µM H2O2 group (p<0.05).\rConclusion: In conclusion, it was observed that silibinin molecule has anti-proliferative\reffect and decreases catalase enzyme activity and MDA level in a concentration dependent\rmanner.en_US
dc.identifier.doi10.5455/annalsmedres.2021.09.558
dc.identifier.endpage508en_US
dc.identifier.issn2636-7688
dc.identifier.issue5en_US
dc.identifier.startpage502en_US
dc.identifier.trdizinid530466en_US
dc.identifier.urihttps://doi.org/10.5455/annalsmedres.2021.09.558
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/530466
dc.identifier.urihttps://hdl.handle.net/20.500.12483/16363
dc.identifier.volume29en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofAnnals of Medical Researchen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleInvestigation of antioxidant effect of silibinin molecule on U-2 OS cells induced by hydrogen peroxideen_US
dc.typeArticleen_US

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