Kronik böbrek hastalığı ile ilişkili anemide lncrna(lincrna-EPS) ekspresyon düzeyinin değerlendirilmesi
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Tarih
2022
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Hatay Mustafa Kemal Üniversitesi
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info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada kronik böbrek hastalığı (KBH) ile ilişkili renal anemisi mevcut olan hastalarda uzun kodlanmayan RNA ailesinden biri olan lincRNA eritroid prosurvivalı'nın (lincRNA-EPS) ekspresyon düzeyleri bakılmasını ve sağlıklı kontrol grubu ile karşılaştırmayı hedefledik. Ayrıca lincRNA-EPS'nin ekspresyon düzeylerinin anemi parametreleri (hemoglobin, MCV, ferritin, vitamin B12, folik asit gibi) ve eritropoetin (EPO) dozları ile ilişkili olup olmadığınının araştırılmasını amaçladık. Gereç ve yöntem: Çalışmamıza hasta sayısı 40, kontrol grubu sayısı ise 19 olmak üzere toplamda 59 kişi dahil edildi. Hastaların demografik özellikleri kaydedildi. Laboratuvar analizleri için kan örnekleri alındı. Alınan kan örneklerinden tam kan sayımı ve biyokimyasal parametreler çalışıldı. LncRNA ekspresyon seviyelerinin analizleri RT-qPCR ile çalışıldı. Evre 3-5 KBH tanısı alan hastalar ve sağlıklı grup şeklinde gruplandırıldı. Veriler SPSS paket programı kullanılarak analiz edildi. Bulgular: LincRNA-EPS ekspresyonu medyan ve kartil değerleri sırasıyla, sağlıklı kontrol grubunda medyan değer: 0,000041; 1. Kartil (Q1): 0,000017, 2. Kartil (Q2): 0,000041, 3. Kartil (Q3): 0,00038, hasta grubunda ise medyan değer: 0,5255; 1. Kartil (Q1): 0,0072, 2. Kartil (Q2): 0,5255, 3. Kartil (Q3): 0,00038 olarak saptandı. Bu veriler değerlendirildiğinde, KBH'ı olan grupta lincRNA-EPS ekspresyon değerleri daha yüksek saptandı (p <0,001). Ayrıca hastaların EPO düzeyleri ile lincRNA-EPS ekspresyon düzeyleri arasında negatif korelasyon bulundu (r=-0,654, p=0,011). Sonuç: Çalışmamız lincRNA-EPS ekspresyon düzeylerinin KBH hastalarında değerlendirildiği literatürdeki ilk çalışmadır. Renal anemisi olan KBH hastalarında, artan lincRNA-EPS ekspresyon düzeyi ve EPO kullanımı ile lincRNA-EPS ekspresyon düzeyinin ters korelasyonu göz önüne alındığında; eritropoezi kolaylaştırmak amacı ile lincRNA-EPS ekspresyon düzeyinin artarak proapoptotik bir gen olan Pycard'ı baskılaması, olası bir mekanizma gibi durmaktadır.
Aim: In this study, we aimed to evaluate the expression levels of lincRNA erythroid prosurvival (lincRNA-EPS), which is one of the long non-coding RNA family, in patients with chronic kidney disease (CKD) related renal anemia and compare it with a healthy control group. We also aimed to investigate whether the expression levels of lincRNA-EPS are associated with anemia parameters (such as hemoglobin, MCV, ferritin, vitamin B12, folic acid) and erythropoietin (EPO) doses. Material and Methods: The total of 59 people were included in our study, with 40 patients and 19 control groups. Demographic characteristics of the patients were recorded. Blood samples were taken for laboratory analysis. Complete blood count and biochemical parameters were studied from the blood samples taken. Analyzes of lincRNA expression levels were studied by RT-qPCR. Patients diagnosed with stage 3-5 CKD and healthy groups were grouped. The data were analyzed using the SPSS package program. Results: The LincRNA-EPS expression median and quartile values were respectively median value: 0.000041; 1st Quartile (Q1): 0.000017, 2nd Quartile (Q2): 0.000041, 3rd Quartile (Q3): 0.00038 in the healthy control group; median value: 0.5255; 1st Quartile (Q1): 0.0072, 2nd Quartile (Q2): 0.5255, 3rd Quartile (Q3): 0.00038 in the patient group. As a result of these data, lincRNA-EPS expression values were found to be higher in the group with CKD (p <0.001). In addition, negative correlation was found between the patients' EPO levels and lincRNA-EPS expression levels (r=-0.654, p=0.011). Conclusion: Our study is the first in the literature to evaluate the levels of lincRNA-EPS in CKD patients. Considering the increased lincRNA-EPS expression level and the inverse correlation between EPO use and lincRNA-EPS expression level in CKD patients with renal anemia; In order to facilitate erythropoiesis, the increase in lincRNA-EPS expression level and suppression of proapoptotic gene Pycard, seems to be a possible mechanism. Key words: Chronic kidney disease, renal anemia, erythropoietin, long non-coding RNA
Aim: In this study, we aimed to evaluate the expression levels of lincRNA erythroid prosurvival (lincRNA-EPS), which is one of the long non-coding RNA family, in patients with chronic kidney disease (CKD) related renal anemia and compare it with a healthy control group. We also aimed to investigate whether the expression levels of lincRNA-EPS are associated with anemia parameters (such as hemoglobin, MCV, ferritin, vitamin B12, folic acid) and erythropoietin (EPO) doses. Material and Methods: The total of 59 people were included in our study, with 40 patients and 19 control groups. Demographic characteristics of the patients were recorded. Blood samples were taken for laboratory analysis. Complete blood count and biochemical parameters were studied from the blood samples taken. Analyzes of lincRNA expression levels were studied by RT-qPCR. Patients diagnosed with stage 3-5 CKD and healthy groups were grouped. The data were analyzed using the SPSS package program. Results: The LincRNA-EPS expression median and quartile values were respectively median value: 0.000041; 1st Quartile (Q1): 0.000017, 2nd Quartile (Q2): 0.000041, 3rd Quartile (Q3): 0.00038 in the healthy control group; median value: 0.5255; 1st Quartile (Q1): 0.0072, 2nd Quartile (Q2): 0.5255, 3rd Quartile (Q3): 0.00038 in the patient group. As a result of these data, lincRNA-EPS expression values were found to be higher in the group with CKD (p <0.001). In addition, negative correlation was found between the patients' EPO levels and lincRNA-EPS expression levels (r=-0.654, p=0.011). Conclusion: Our study is the first in the literature to evaluate the levels of lincRNA-EPS in CKD patients. Considering the increased lincRNA-EPS expression level and the inverse correlation between EPO use and lincRNA-EPS expression level in CKD patients with renal anemia; In order to facilitate erythropoiesis, the increase in lincRNA-EPS expression level and suppression of proapoptotic gene Pycard, seems to be a possible mechanism. Key words: Chronic kidney disease, renal anemia, erythropoietin, long non-coding RNA
Açıklama
Anahtar Kelimeler
İç Hastalıkları, Internal diseases, Kronik böbrek hastalığı, renal anemi, eritropoetin, uzun kodlanmayan RNA
