Rat karaciğerinde metotreksat ile oluşturulan oksidatif stres üzerine rutinin etkisi
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Dosyalar
Tarih
2020
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Sunulan çalışmada, rutinin rat karaciğerinde metotreksat ile oluşturulan oksidatif stres üzerine olası koruyucu etkilerinin araştırılması amaçlandı. Çalışmada 24 adet, Wistar Albino rat (2 aylık, erkek) ile Kontrol (K), Metotreksat (M), Rutin (R), Metotreksat ve Rutin (MR) olmak üzere dört grup oluşturuldu. K ve M gruplarına distile su, R ve MR gruplarına ise rutin 50 mg/kg/gün oral gavaj yoluyla on beş gün boyunca verildi. Ayrıca, çalışmanın sekizinci gününde M ve MR gruplarına metotreksat 20 mg/kg, K ve R gruplarına serum fizyolojik (% 0,9'luk) periton içi (i.p.) uygulandı. Çalışmanın on altıncı gününde karaciğer dokuları çıkarıldı. Karaciğer dokularında malondialdehit (MDA), vitamin C, total glutatyon (tGSH), nikotinamid adenin dinükleotit fosfat (NADPH) düzeyleri ile glutatyon peroksidaz (GPx) ve glukoz-6-fosfat dehidrojenaz (G6PD) aktiviteleri belirlendi. M grubunda, diğer gruplara göre MDA düzeyinde artış ve vitamin C düzeylerinde azalış istatistiksel olarak önemli bulundu (p<0,001). M ve MR gruplarında tGSH düzeyinin K grubuna kıyasla düşük olduğu gözlendi (p<0,05). M grubu NADPH düzeylerinde ve GPx aktivitesinde, K grubuna göre önemli düzeyde azalış olduğu belirlendi (p<0,001). R grubu NADPH düzeyi M grubuna göre yüksek, K grubuna göre düşük bulundu (p<0,001). Ayrıca R ve MR grupları GPx aktiviteleri K ve M gruplarına kıyasla anlamlı derecede düşük olduğu belirlendi (p<0,001). Bu çalışmada, metotreksatın karaciğer dokusunda oksidatif hasara yol açtığı ve rutinin metotreksat kaynaklı hepatik hasar üzerinde koruyucu ve iyileştirici etkileri olduğu belirlendi. Sonuç olarak, rutinin kemoterapötik amaçla uygulanan metotreksatın yan etkilerini azaltmada yararlı olabileceği öngörüldü
In this study, it was aimed to investigate the possible protective effects of rutin on methotrexate-induced oxidative hepatic damage in rat. Twenty-four Wistar Albino rats (2 months old, male) were divided randomly into four groups (n = 6) as Control (C), Methotrexate (M), Rutin (R), Methotrexate and Rutin (MR). C and M received distilled water, R and MR groups received rutin (50 mg/kg/day), orally, for fifteen days. M and MR groups were administered methotrexate (20 mg/kg) intraperitoneally (i.p.) and C and R groups were administered saline (0.9 %) i.p. on the eighth day of the study. Rats were sacrificed at the sixteenth day and liver tissues were removed. The levels of malondialdehyde (MDA), vitamin C, total glutathione (tGSH) and the activities of glutathione peroxidase (GPx) and glucose-6-phosphate dehydrogenase (G6PD) were determined colorimetrically. MDA levels increased and vitamin C levels decreased in the M group compared to other groups (p<0.001). tGSH levels reduced in both M and MR groups compared to C group (p<0.05). A significant decrease in M group NADPH levels and GPx activity compared to C group (p<0.001) was observed. NADPH levels of R group were found to be higher than M group and lower than C group (p<0.001). In addition, it was determined that the activities of GPx in R and MR groups were lower than C and M groups (p<0.001). In this study, it was determined that methotrexate caused oxidative damage in liver and rutin had protective and ameliorating effects on methotrexate-induced hepatic damage. Consequently, it may be suggested that rutin may be useful in reducing the side effects of methotrexate, applied for chemotherapy.
In this study, it was aimed to investigate the possible protective effects of rutin on methotrexate-induced oxidative hepatic damage in rat. Twenty-four Wistar Albino rats (2 months old, male) were divided randomly into four groups (n = 6) as Control (C), Methotrexate (M), Rutin (R), Methotrexate and Rutin (MR). C and M received distilled water, R and MR groups received rutin (50 mg/kg/day), orally, for fifteen days. M and MR groups were administered methotrexate (20 mg/kg) intraperitoneally (i.p.) and C and R groups were administered saline (0.9 %) i.p. on the eighth day of the study. Rats were sacrificed at the sixteenth day and liver tissues were removed. The levels of malondialdehyde (MDA), vitamin C, total glutathione (tGSH) and the activities of glutathione peroxidase (GPx) and glucose-6-phosphate dehydrogenase (G6PD) were determined colorimetrically. MDA levels increased and vitamin C levels decreased in the M group compared to other groups (p<0.001). tGSH levels reduced in both M and MR groups compared to C group (p<0.05). A significant decrease in M group NADPH levels and GPx activity compared to C group (p<0.001) was observed. NADPH levels of R group were found to be higher than M group and lower than C group (p<0.001). In addition, it was determined that the activities of GPx in R and MR groups were lower than C and M groups (p<0.001). In this study, it was determined that methotrexate caused oxidative damage in liver and rutin had protective and ameliorating effects on methotrexate-induced hepatic damage. Consequently, it may be suggested that rutin may be useful in reducing the side effects of methotrexate, applied for chemotherapy.
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry ; Veteriner Hekimliği
