Hatay ve Gaziantep illerindeki bağ alanlarında görülen önemli vi̇rüs hastalıklarının serolojik ve moleküler yöntemlerle tanılanması
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Dosyalar
Tarih
2015
Yazarlar
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Günümüzde bağları enfekte eden 60 kadar virüs saptanmıĢtır. Bu virüslerin hemen hemen yarısı Nepovirus, Sadwavirus, Closterovirus, Ampelovirus, Vitivirus, Maculavirus ve Foveavirus cinsine ait virüslerdir. Bu cinsler içinde yer alan virüslerin bazıları ülkemizin farklı bölgelerinde tespit edilmiĢ olup bu çalıĢmaların çoğu Trakya, Ege, Ġç Anadolu ve Güneydoğu Anadolu bölgelerinde biyolojik ve serolojik yöntemler kullanılarak yapılmıĢtır. Moleküler tanı yöntemlerinin bağ virüslerini tanılamak amacıyla kullanımı ise ülkemizde son yıllarda baĢlamıĢ olup, Hatay ili bağlarında virüs hastalıklarının moleküler yöntemlerle teĢhisine yönelik hiçbir çalıĢma bulunmamaktadır. Bu çalıĢmanın amacı Hatay ve Gaziantep illerindeki bağ alanlarında Closteroviridae familyasına ait virüslerden Grapevine leafroll associated virus 1, 2, 3, 4, 5, 6, 7 ve 9 (GLRaV-1, -2, -3, -4, -5, -6, -7, -9) ile özellikle odun dokusunda deformasyonlara neden olan Grapevine virus A (GVA), Grapevine virus B (GVB) ve Grapevine virus D (GVD)'nin DAS-ELISA ve RT-PCR yöntemleri ile; ülkemizde hiç çalıĢma bulunmayan ve son yıllarda tespit edilen "Red blotch associated virus (GRBaV) 'ün de PCR analizleri ile saptanmasıdır. Bu çalıĢma kapsamında Hatay ilinde 11, Gaziantep ilinde ise 2 bağ ziyaret edilmiĢ ve 153 bitki örneği toplanmıĢtır. Gözlenen en yaygın simptomlar yapraklarda kızarma ve damarların yeĢil kalması, beneklenme, kıvrılmalar ve Ģekil bozuklukları, sürgünlerde ise boğum aralarında kısalmalar ile odun gövdesinde deformasyonlardır. ELISA testi sonuçlarına göre en yaygın virüsler GLRaV-1 (%55.56) , GLRaV 4-9 (%43.14), GLRaV-2 (%15.69), ve GLRaV-3 (%12.42) olarak saptanmıĢ ve bu virüsleri GVA (%4.57) ve GLRaV-6 (%0.65) izlemiĢtir. Testlenen tüm örnekler GLRaV- 5, GLRaV-7 ve GVB açısından negatif bulunmuĢtur. KarıĢık enfeksiyonların hepsinde GLRaV-1'in yer aldığı, bunu sırasıyla GLRaV 4-9, GLRaV-2, GLRaV-3, GLRaV-6 ve GVA'nın izlediği görülmüĢtür. RTPCR analizleri sonucunda testelenen bağ örneklerinin ise % 23.94'ü GLRaV-1, %14.77'si GLRaV-3, % 15.38'i GLRaV-6, %13.64'ü GVA, %1.26'sı GVB ve %1.20'si GVD ile enfekteli bulunmuĢtur. Bu çalıĢma ile testlenen 153 örneğin hiç birisinde GRBaV tespit edilemezken GVB ve GVD virüsleri ülkemizde ilk kez RT-PCR yöntemi ile tespit edilmiĢtir. 2015, 45 sayfa
To date, nearly 60 viruses have been reported to infect grapevines. Of these viruses, only about half, belonging primarily to the genera Nepovirus, Sadwavirus, Closterovirus, Ampelovirus, Vitivirus, Maculavirus and Foveavirus. Some of the viruses among these genera have already been detected in Trace, Agean, Central and Southeast regions of Turkey by using biological and serological methods. Molecular detection tools have been recently used in our country for grapevine viruses but there has been no report on molecular detection of any grapevine viruses in Hatay province, yet. The aim of this study is detection of Grapevine leafroll associated virus 1, 2, 3, 4, 5, 6, 7, 9 (GLRaV-1, 2,3, 4,5, 6, 7, 9) which belong to Closteroviridae, Grapevine virus A (GVA), Grapevine virus B (GVB) and Grapevine virus D (GVD) which cause wood deformations on grapevines by DAS-ELISA and RT-PCR and as well as "Red blotch associated virus (GRBaV) on which there is no any report in Turkey by PCR. In frame of this study, 11 vineyards in Hatay and 2 vineyards in Gaziantep were visited and 153 samples were collected.The most common symptoms were reported as reddining of the leaves and green veins, mottling, leaf rolling, leaf and wood deformations and short internodes. According to ELISA results, most common viruses were GLRaV-1 (55.56%) , GLRaV 4-9 (43.14%), GLRaV-2 (15.69%), GLRaV-3 (12.42%) and followed by GVA (4.57%) and GLRaV-6 (%0.65). All tested samples, were found negative for GLRaV- 5, GLRaV-7 and GVB. GLRaV-1 placed in all mixedinfection combinations and followed by GLRaV 4-9, GLRaV-2, GLRaV-3, GLRaV-6 and GVA. RT-PCR analysis showed that tested samples were infected by GLRaV-1, GLRaV-6, GLRaV-3, , GVA, GVB and GVD as 23.94%, 15.38%, 14.77%, 13.64%, %1.26, 1.20%, respectively. In this study GRBaV was not able to detected in any of the 153 tested samples and also GVB and GVD were first time detected by RT-PCR in Turkey. 2015, 45 pages Key words: Vitis vinifera L., Closteroviridae, Vitivirus, Red blotch associated virus, DAS-ELISA, PCR, Hatay, Gaziantep.
To date, nearly 60 viruses have been reported to infect grapevines. Of these viruses, only about half, belonging primarily to the genera Nepovirus, Sadwavirus, Closterovirus, Ampelovirus, Vitivirus, Maculavirus and Foveavirus. Some of the viruses among these genera have already been detected in Trace, Agean, Central and Southeast regions of Turkey by using biological and serological methods. Molecular detection tools have been recently used in our country for grapevine viruses but there has been no report on molecular detection of any grapevine viruses in Hatay province, yet. The aim of this study is detection of Grapevine leafroll associated virus 1, 2, 3, 4, 5, 6, 7, 9 (GLRaV-1, 2,3, 4,5, 6, 7, 9) which belong to Closteroviridae, Grapevine virus A (GVA), Grapevine virus B (GVB) and Grapevine virus D (GVD) which cause wood deformations on grapevines by DAS-ELISA and RT-PCR and as well as "Red blotch associated virus (GRBaV) on which there is no any report in Turkey by PCR. In frame of this study, 11 vineyards in Hatay and 2 vineyards in Gaziantep were visited and 153 samples were collected.The most common symptoms were reported as reddining of the leaves and green veins, mottling, leaf rolling, leaf and wood deformations and short internodes. According to ELISA results, most common viruses were GLRaV-1 (55.56%) , GLRaV 4-9 (43.14%), GLRaV-2 (15.69%), GLRaV-3 (12.42%) and followed by GVA (4.57%) and GLRaV-6 (%0.65). All tested samples, were found negative for GLRaV- 5, GLRaV-7 and GVB. GLRaV-1 placed in all mixedinfection combinations and followed by GLRaV 4-9, GLRaV-2, GLRaV-3, GLRaV-6 and GVA. RT-PCR analysis showed that tested samples were infected by GLRaV-1, GLRaV-6, GLRaV-3, , GVA, GVB and GVD as 23.94%, 15.38%, 14.77%, 13.64%, %1.26, 1.20%, respectively. In this study GRBaV was not able to detected in any of the 153 tested samples and also GVB and GVD were first time detected by RT-PCR in Turkey. 2015, 45 pages Key words: Vitis vinifera L., Closteroviridae, Vitivirus, Red blotch associated virus, DAS-ELISA, PCR, Hatay, Gaziantep.
Açıklama
Anahtar Kelimeler
Ziraat, Agriculture, Vitis vinifera L., Closteroviridae, Vitivirus, Red blotch associated virus, DAS-ELISA, PCR, Hatay, Gaziantep.
