Diyabet oluşturulan ratların böbrek dokusunda oksidatif stres ve apoptozis üzerine perilil alkolün koruyucu etkileri
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Fırat Üniversitesi
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Bu çalışmada diyabet oluşturulan ratların böbrek dokusunda oksidatif stres ve apoptozis üzerine perilil alkolün etkileri araştırılmıştır. Bu amaçla 40 adet rat eşit olarak rastgele 4 gruba ayrılmıştır. 1.Grup (kontrol), 2. Grup (Diyabet; STZ 50 mg/kg i.p, tek doz), 3. Grup (Perilil Alkol; Perilil Alkol 50 mg/kg/gün p.o, 7 gün), 4. Grup (Diyabet+Perilil Alkol; STZ 50 mg/kg i.p, tek doz+Perilil Alkol 50 mg/kg/gün, p.o 7 gün). Çalışma sonunda böbrek dokusu örneklerinde bazı biyokimyasal parametreler malondialdehit (MDA), glutatyon (GSH), glutatyon peroksidaz (GSH.Px), katalaz (CAT) ve apoptotik indeks markerları olan Bax ve Bcl-2 seviyeleri Western blot analizleri ile ölçülmüştür. Çalışma sonucunda diyabet oluşturulan grupta MDA seviyesi kontrol grubuna göre artmış (P<0.01); GSH seviyesi, GSH-Px ve CAT aktiviteleri ise azalmıştır (P<0.05). Diyabet grubuyla kıyaslandığında, Diyabet+Perilil Alkol grubunda MDA düzeyi azalmış (P<0.01); GSH, GSH-Px ve CAT aktiviteleri ise yükselmiştir (P<0.05). Bax protein düzeyleri Diyabet grubunda kontrol grubuna göre artış göstermiş; fakat Diyabet+Perilil Alkol grubunda azalmıştır (P<0.05). Bcl2 seviyesi ise Diyabet+Perilil Alkol grubunda artış göstermiştir (P<0.05). Bax/Bcl-2 oranı ise Diyabet grubunda artmış olmasına rağmen (P<0.05), Diyabet+Perilil Alkol grubunda azalmıştır. Sonuç olarak, perilil alkolün diyabetin erken döneminde oluşan oksidatif stresi azalttığı ve antioksidan etkinliği artırdığı, bununla birlikte apoptotik indeks göstergesi olan Bax/Bcl-2 oranını azalttığı tespit edilmiştir.
The aim of this study was to investigate the effects of perilyl alcohol on renal tissue oxidative stress and apoptosis in diabetic rats. For this purpose, 40 rats were divided into 4 groups with 10 rats per group. The experimental groups were Group 1 (control), Group 2 (Diabetes; STZ 50 mg/kg ip, Single Dose), Group 3 (Perilil Alcohol; Perilil Alcohol 50 mg/kg po, 7 days), and Group 4 (Diabetes+Perilil Alcohol; STZ 50 mg/kg ip, Single Dose+Perilyl Alcohol 50 mg/kg po 7 days). Biochemical parameters malondialdehyde (MDA), Glutathione (GSH), Glutathione Peroxidase (GSH.Px), Catalase (CAT) and Bax and Bcl-2 levels were measured at the end of the study. Results showed that MDA level increased in diabetic group compared to control group (P<0.01); GSH, GSH-Px and CAT levels decreased (P<0.05). In the diabetes + perilyl alcohol group; Compared with the diabetes group, MDA level was decreased (P<0.01); GSH, GSH-Px and CAT levels increased (P<0.05). As a result of Western blot analysis, Bax protein levels increased in Diabetes group; but decreased in the Diabetes+Perilyl Alcohol group (P<0.05). Bcl-2 level increased in Diabetes+Perilyl Alcohol group (P<0.05). Although Bax/Bcl-2 ratio increased in Diabetes group (P<0.05), it decreased in Diabetes+Perilyl Alcohol group. As a result, it was found that perilyl alcohol decreased oxidative stress and increased antioxidant activity in early stage of diabetes, but also decreased Bax / Bcl-2 ratio which is an indicator of apoptotic index.
The aim of this study was to investigate the effects of perilyl alcohol on renal tissue oxidative stress and apoptosis in diabetic rats. For this purpose, 40 rats were divided into 4 groups with 10 rats per group. The experimental groups were Group 1 (control), Group 2 (Diabetes; STZ 50 mg/kg ip, Single Dose), Group 3 (Perilil Alcohol; Perilil Alcohol 50 mg/kg po, 7 days), and Group 4 (Diabetes+Perilil Alcohol; STZ 50 mg/kg ip, Single Dose+Perilyl Alcohol 50 mg/kg po 7 days). Biochemical parameters malondialdehyde (MDA), Glutathione (GSH), Glutathione Peroxidase (GSH.Px), Catalase (CAT) and Bax and Bcl-2 levels were measured at the end of the study. Results showed that MDA level increased in diabetic group compared to control group (P<0.01); GSH, GSH-Px and CAT levels decreased (P<0.05). In the diabetes + perilyl alcohol group; Compared with the diabetes group, MDA level was decreased (P<0.01); GSH, GSH-Px and CAT levels increased (P<0.05). As a result of Western blot analysis, Bax protein levels increased in Diabetes group; but decreased in the Diabetes+Perilyl Alcohol group (P<0.05). Bcl-2 level increased in Diabetes+Perilyl Alcohol group (P<0.05). Although Bax/Bcl-2 ratio increased in Diabetes group (P<0.05), it decreased in Diabetes+Perilyl Alcohol group. As a result, it was found that perilyl alcohol decreased oxidative stress and increased antioxidant activity in early stage of diabetes, but also decreased Bax / Bcl-2 ratio which is an indicator of apoptotic index.
Description
Keywords
Diyabet, Perilil alkol, Apoptozis, Oksidatif stres, Böbrek, Diabetes, Perilyl alcohol, Apoptosis, Oxidative stress, Kidney
Journal or Series
Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
WoS Q Value
Scopus Q Value
Volume
34
Issue
1
Citation
YILMAZ M,AKSAKAL M,GÜVENÇ M,ARKALI G (2020). Diyabet Oluşturulan Ratların Böbrek Dokusunda Oksidatif
Stres ve Apoptozis Üzerine Perilil Alkolün Koruyucu Etkileri. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 34(1), 29 - 34.
