ß-amiloid ile indüklenen ın vitro alzheimer hastalığı modelinde ß-glisiretinik asit'in koruyucuetkinliğinin araştırılması
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Tarih
2020
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Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışma, U87 Malign Glioma (MG) hücre hattında amiloid ß1-40 (Aß) ile indüklenen in vitro Alzheimer Hastalığı (AH) modelinde apoptotik yolaklar ve antioksidan enzimler üzerine meyan kökü (Glycyrrhiza glabra) bitki ekstresinden elde edilen 18ß- Glisiretinik asitin (GA) koruyucu etkilerini araĢtırmak amacıyla gerçekleştirildi. Çalışmada U87 MG hücre hattından ibaret kontrol grubu, Aß ile indüklenmiĢ U-87 hücre hatlarını içeren in vitro AH modelinin oluĢturulduğu Aβ grubu ve Aβ grubuna 18ß GA eklenmesiyle oluĢturulan Aß+18ß GA grubu olmak üzere 3 grup oluşturuldu. Apoptozisin göstergesi olarak kaspaz-3, antioksidan durumun belirlenebilmesi için de süper oksit dismutaz (SOD), katalaz (KAT) ve glutatyon peroksidaz (GPx) enzimlerinin seviyeleri analiz edildi. Kontrol grubuna kıyaslandığında Aß grubunda kaspaz-3 seviyesi istatiksel olarak önemli şekilde yüksek bulunurken; Aß+18ß GA grubunda ise Aß grubuna kıyasla kaspaz-3 seviyesi istatiksel olarak anlamlı şekilde düşük tespit edildi (p<0,05). Bununla birlikte, kontrol grubu ile kıyaslandığında Aß grubunda SOD, KAT ve GPx enzim seviyeleri istatiksel olarak anlamlı şekilde düşük bulunurken, Aß+18ß GA grubunda Aß grubuna kıyasla bu enzim seviyeleri istatiksel olarak anlamlı şekilde yüksek tespit edildi (p<0,05). Sonuç olarak, Aß ile indüklenmiş in vitro AH modelinde 18ß-GA'nın antioksidan enzim düzeylerini artırarak ve apoptotik yolakları aktive ederek koruyucu bir potansiyele sahip olduğu ortaya konuldu. Bu etkiler göz önüne alındığında 18ß-GA'nın AH'da koruyucu ya da alternatif tedavide kullanım potansiyeline sahip olabileceği, bunun yapılacak daha ileri çalışmalarla desteklenmesi gerektiği değerlendirildi.
This study was carried out to investigated the protective effects of 18ß- Glycyrretinic acid (GA) which is derived from licorice root (Glycyrrhiza glabra) plant extract on apoptotic pathways and antioxidant enzymes in an in vitro Alzheimer's Disease (AD) model induced by amyloid ß1-40 (Aß) in U87 Malignant Glioma cell line. In the study, three groups were formed: the control group consisting of U87 MG cell line, the Aβ group, in which the in vitro AH model was created containing the Aß- induced U-87 cell lines , and the Aß + 18ß GA group created by adding 18ß GA to the Aβ group. Caspase-3 as an indicator of apoptosis ,and to be able to determine the antioxidant status the levels of super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes were analyzed. When compared to the control group, the caspase-3 level in the Aß group was found to be statistically significantly higher; In the Aß +18ß GA group, the caspase-3 level was determined statistically significantly lower compared to the Aß group (p <0.05). Nevertheless, when compared to the control group, SOD, CAT and GPx enzyme levels were found statistically significantly low in the Aß group, while these enzyme levels were determined to be statistically considerably high in the Aß + 18ß GA group compared to the Aß group (p <0.05). As a result, it was revealed that 18ß-GA has a protective potential by increasing antioxidant enzyme levels and activating apoptotic pathways in Aß-induced in vitro AD model.When these effects are considered, it was evaluated that 18ß-GA could have the potential to be used in preventive or alternative treatment in AD, and this should be supported by further studies.
This study was carried out to investigated the protective effects of 18ß- Glycyrretinic acid (GA) which is derived from licorice root (Glycyrrhiza glabra) plant extract on apoptotic pathways and antioxidant enzymes in an in vitro Alzheimer's Disease (AD) model induced by amyloid ß1-40 (Aß) in U87 Malignant Glioma cell line. In the study, three groups were formed: the control group consisting of U87 MG cell line, the Aβ group, in which the in vitro AH model was created containing the Aß- induced U-87 cell lines , and the Aß + 18ß GA group created by adding 18ß GA to the Aβ group. Caspase-3 as an indicator of apoptosis ,and to be able to determine the antioxidant status the levels of super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes were analyzed. When compared to the control group, the caspase-3 level in the Aß group was found to be statistically significantly higher; In the Aß +18ß GA group, the caspase-3 level was determined statistically significantly lower compared to the Aß group (p <0.05). Nevertheless, when compared to the control group, SOD, CAT and GPx enzyme levels were found statistically significantly low in the Aß group, while these enzyme levels were determined to be statistically considerably high in the Aß + 18ß GA group compared to the Aß group (p <0.05). As a result, it was revealed that 18ß-GA has a protective potential by increasing antioxidant enzyme levels and activating apoptotic pathways in Aß-induced in vitro AD model.When these effects are considered, it was evaluated that 18ß-GA could have the potential to be used in preventive or alternative treatment in AD, and this should be supported by further studies.
Açıklama
Anahtar Kelimeler
Eczacılık ve Farmakoloji, Pharmacy and Pharmacology
