A research on Babesia and Theileria species in sheep and goats of Kirikkale province through molecular methods

Basit Öğe Kaydı
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.contributor.authorGokpinar, Sami
dc.contributor.authorGazyagci, Aycan Nuriye
dc.contributor.authorAydenizoz, Meral
dc.contributor.authorKaya, Ufuk
dc.date.accessioned2024-09-18T20:02:50Z
dc.date.available2024-09-18T20:02:50Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kirikkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with a length of 360 to 430 bp on variable region V4 of the 18 ssu rRNA gene of Theileria and Babesia species were proliferated; PCR products obtained were hybridized on a membrane where species-specific probes. Piroplasms were detected in 54 (18%) sheep and in none of the goats during microscopic examination of blood smears. With the RLB technique; T. ovis 70%, Theileria sp. 2.3%, B. ovis 5.3%, T. ovis + B. ovis 2.7% were detected in sheep. In goats; T. ovis was detected at a rate of 1%. Consequently, this study is the first for detection of Theileria and Babesia species in sheep and goats in Kirikkale. Presence of T. ovis and B. ovis in the sheep, and T. ovis in goats was detected in Kirikkale.en_US
dc.description.sponsorshipKirikkale University Scientific Research Coordination Unit [2014/119]en_US
dc.description.sponsorshipThis research was supported by the Kirikkale University Scientific Research Coordination Unit (Project number: 2014/119).en_US
dc.identifier.doi10.3906/vet-2103-17
dc.identifier.endpage919en_US
dc.identifier.issn1300-0128
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85118794737en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage912en_US
dc.identifier.trdizinid528190en_US
dc.identifier.urihttps://doi.org/10.3906/vet-2103-17
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/528190
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8049
dc.identifier.volume45en_US
dc.identifier.wosWOS:000711273500014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBabesiaen_US
dc.subjectgoaten_US
dc.subjectRLBen_US
dc.subjectsheepen_US
dc.subjectTheileriaen_US
dc.titleA research on Babesia and Theileria species in sheep and goats of Kirikkale province through molecular methodsen_US
dc.typeArticleen_US

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