BVDV monitoring by pooling and real time RT-PCR as economical monitoring technique with low BVDV prevalence

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dc.contributor.authorÜn, Hikmet
dc.contributor.authorGökçe, Mustafa Murat
dc.contributor.authorAyaz, Oğuz
dc.contributor.authorSimsek, Suna
dc.contributor.authorKarabulut, Osman
dc.date.accessioned2024-09-19T16:23:37Z
dc.date.available2024-09-19T16:23:37Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of this study was to determine whether the use of pooled blood samples and real-time RT-PCR are feasible for detecting BVDV in viremic or PI animals. For this purpose, blood samples obtained from 2701 cattle, brought from 62 different farms, were used to test for the presence of bovine viral diarrhoea virus (BVDV) at the Veterinary Virology Department of Aksaray University Faculty of Veterinary Medicine. The sampling was done from all geographical regions in Turkey. Blood samples were pooled in groups of eight, creating a total of 342 pools. Each pool was checked for BVDV with a real-time RT-PCR test. BVDV nucleic acid was detected in 18 (5.26%) of a total of 342 pools. BVDV was detected in 18 of 62 farms. The most important result obtained from this study is that BVDV monitoring by pooling and real time RT-PCR can be done very economically when the disease prevalence is low (<10%). A risk/benefit estimation can be done for breeders who want to start vaccination programs.en_US
dc.identifier.doi10.31797/vetbio.1072218
dc.identifier.endpage79en_US
dc.identifier.issn2548-1150
dc.identifier.issue1en_US
dc.identifier.startpage72en_US
dc.identifier.trdizinid1122807en_US
dc.identifier.urihttps://doi.org/10.31797/vetbio.1072218
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1122807
dc.identifier.urihttps://hdl.handle.net/20.500.12483/16005
dc.identifier.volume7en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofJournal of advances in vetbio science and techniquesen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBVDVen_US
dc.subjectPoolingen_US
dc.subjectReal Time RT-PCRen_US
dc.titleBVDV monitoring by pooling and real time RT-PCR as economical monitoring technique with low BVDV prevalenceen_US
dc.typeArticleen_US

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