Behçet hastalarında oksidatif stress ve antioksidan savunma mekanizması
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Tarih
2011
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info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı Behçet Hastalığı’nın aktif ve inaktif dönemlerinde oksidan /antioksidan sistemin durumunu değerlendirmek için serum malondialdehid, nitrik oksit seviyeleri ile glutatiyon peroksidaz ve süperoksit dismutaz enzim aktivitelerinin ölçülmesidir. Gereç ve Yöntem: Çalışmamıza Behçet Hastalığı tanısı almış 22 hasta dahil edildi. Klinik değerlendirmede klinik semptomlarda kötüleşme ve üveiti olan hastalar aktif dönem olarak değerlendirildi. Nitrik oksidin in vitro ve in vivo üretiminin göstergesi olan nitrit miktarı Griess reaksiyonu ile ölçüldü. Serum malondialdehid seviyesi, malondialdehidin tiyobarbitürik asit ile 90-100 oC’de reaksiyona girmesine dayanan bir metot ile ölçüldü. Toplam süperoksit dismutaz aktivitesi ölçümü, xanthine/xanthine oksidasyon sistemi ile elde edilen O2 ile nitroblue tetrozolyumun indirgenmesinin inhibisyonuna dayanan yöntem ile ölçüldü. Glutatiyon peroksida Bulgular: Aktif dönemdeki malondialdehid (aktif: 2,927 ± 0,292 µmol / L, inaktif: 2,145 ± 0,188 µmol / L)ve nitrik oksit (aktif: 115,03 ± 3,28 µmol / L , inaktif: 78,20 ± 5,33 µmol / L) seviyeleri anlamlı olarak yüksek bulundu(p<0,05). Glutatiyon peroksidaz (aktif: 89,170 ± 5,83 U / L, inaktif: 140,99 ± 11,10 U / L) ve süperoksit dismutaz (aktif: 6,094 ± 0,467 U / L, inaktif: 7,688 ± 0,429 U / L) aktivitelerinde ise aktif dönemde inaktif dönem ile kıyaslandığında anlamlı azalma tespit edildi (p<0,05) Sonuç: Bu bulgular Behçet Hastalığı’nda oksidan/antioksidan dengenin bozulduğunu ve oksidatif stres olarak adlandırılan durumun oluştuğunu desteklemektedir. Behçet Hastalığı'nın şiddeti de bozulmuş olan antioksidan mekanizmaya bağlı olabilir. Bu bulgular hastalığın patogenezi ve prognozu ile ilgili bilgilere katkı sağlamaktadır ve konu ile ilgili yeni çalışmalara olan ihtiyacı göstermektedir.
Aim: The purpose of this study was to evaluate the serum malondialdehyde, nitric oxide levels, glutathione peroxidase and superoxide dismutase activities in patients with Behcet’s Disease both in their active and inactive periods to assess the oxidant/antioxidant status. Material and Methods: Twenty two patients with Behçet’s disease were included to the study. Patients with worsening of clinical symptoms along with having uveitis were considered to be in active period. Quantitation of nitrite, which is an indicator of nitric oxide in vivo and in vitro, is based on Griess reaction. Serum malondialdehyde concentrations were determined by a method based on the reaction with thiobarbituric acid. Total superoxide dismutase activity was determined by the inhibition of nitroblue tetrazolium reduction by O2 generated by the xanthine/ xanthine oxidizing system. Glutathione peroxidase activity was measured with the method of Paglia and Valentine. Results: Malondialdehyde activity (active: 2,927 ± 0,292 µmol / L, inactive: 2,145 ± 0,188 µmol / L) and nitric oxide (active: 115,03 ± 3,28 µmol / L , inactive: 78,20 ± 5,33 µmol / L) levels in active period were increased significantly (p<0,05). Whereas, the activities of glutathione peroxidase (active: 89,170 ± 5,83 U / L, inactive: 140,99 ± 11,10 U / L) and superoxide dismutase (active: 6,094 ± 0,467 U / L, inactive: 7,688 ± 0,429 U / L) were decreased significantly in active period (p<0,05). Conclusion: Our findings confirm the presence of deteriorated oxidant/antioxidant equilibrium in favour of oxidative stress. The severity of Behçet’s disease may be arise from impaired antioxidant mechanisms.
Aim: The purpose of this study was to evaluate the serum malondialdehyde, nitric oxide levels, glutathione peroxidase and superoxide dismutase activities in patients with Behcet’s Disease both in their active and inactive periods to assess the oxidant/antioxidant status. Material and Methods: Twenty two patients with Behçet’s disease were included to the study. Patients with worsening of clinical symptoms along with having uveitis were considered to be in active period. Quantitation of nitrite, which is an indicator of nitric oxide in vivo and in vitro, is based on Griess reaction. Serum malondialdehyde concentrations were determined by a method based on the reaction with thiobarbituric acid. Total superoxide dismutase activity was determined by the inhibition of nitroblue tetrazolium reduction by O2 generated by the xanthine/ xanthine oxidizing system. Glutathione peroxidase activity was measured with the method of Paglia and Valentine. Results: Malondialdehyde activity (active: 2,927 ± 0,292 µmol / L, inactive: 2,145 ± 0,188 µmol / L) and nitric oxide (active: 115,03 ± 3,28 µmol / L , inactive: 78,20 ± 5,33 µmol / L) levels in active period were increased significantly (p<0,05). Whereas, the activities of glutathione peroxidase (active: 89,170 ± 5,83 U / L, inactive: 140,99 ± 11,10 U / L) and superoxide dismutase (active: 6,094 ± 0,467 U / L, inactive: 7,688 ± 0,429 U / L) were decreased significantly in active period (p<0,05). Conclusion: Our findings confirm the presence of deteriorated oxidant/antioxidant equilibrium in favour of oxidative stress. The severity of Behçet’s disease may be arise from impaired antioxidant mechanisms.
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Genel ve Dahili Tıp
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Tıp Araştırmaları Dergisi
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Cilt
9
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3
