Kronik tenofovir uygulaması sonucu ortaya çıkabilecek olan nefrotoksisite, hepatotoksisite, nörotoksisite ve pankreas hasarı üzerine kafeik asit fenetil ester (CAPE)'in koruyucu etkilerinin araştırılması
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Dosyalar
Tarih
2014
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Kronik tenofovir uygulaması sonucu ortaya çıkabilecek olan nefrotoksisite, hepatotoksisite, nörotoksisite ve pankreas hasarı üzerine kafeik asit fenetil ester (CAPE)'nin koruyucu etkilerinin araştırılması amacıyla yapılmıştır. Yöntem: Çalışma 200-300 gr ağırlığındaki erişkin Wistar Albino dişi cinsi 21 adet sıçan üzerinde yapıldı. Ratlar randomize şekilde kontrol (C), tenofovir (T), CAPE, tenofovir+CAPE olmak üzere 4 gruba ayrıldı. Çalışmada T ratlara kronik olarak 30 gün boyunca 30 mg/kg/gün oral yolla verildi. Sonra intraperitonel olarak CAPE 10 µmol/kg dozunda uygulandı. Biyokimyasal ve histopatolojik değerlendirme yapıldı. Bulgular: Histomorfolojide; T grubunda pankreasta hasar oluştuğu, T+CAPE grubunda ise hasarın azaldığı ve beta hücre yoğunluğunun kontrol grubuna göre T grubunda azalırken, T+CAPE grubunda sadece T verilen gruba göre arttığı belirlendi. Böbrek dokusunda; T'nin artan apopitoza ve tübüler toksisiteye neden olduğu, ancak T+CAPEkullanımının ise tübüler toksisiteyi engellediği belirlendi. Beyinde; T grubunda inflamasyon, vakuoler dejenerasyon ve nekrobiotik değişiklikler gözlenirken, yine T+CAPE grubunda ise ılımlı bir düzelme izlendi. Karaciğerde; T grubunda hasar oluştuğu, T+CAPE grubunda ise apopitoz düzeyinin sadece T verilen gruba göre düştüğü ve rejeneratif değişiklikler ve fokal sitoplazmik hipereozinofiligözlendi. Biyokimyasal açıdan; T grubunda üre ve kreatinin değerlerinin diğer K, CAPE, T+CAPE gruplarına göre anlamlı olmasada yüksek düzeyde olduğu ve CAPE'nin T'nin yükselttiği özellikle kreatinin seviyelerini azalttığı belirlendi. Histopatolojik bulguları biyokimyasal bulgular doğruladı. Sonuç: Kronik T kullanımının karaciğer, beyin, pankreas ve böbrek dokularında hasara neden olduğu, ancak tenofovir sonrası CAPE kullanımının; T toksisitesini azaltabildiği belirlendi. Buna göre, CAPE'nin antiviral tedavide profilaktif amaçla kullanımı konusunda daha uzun süreli çalışmaların yapılması gerektiği sonucuna varıldı.
Purpose: To investigation of protective effects of caffeic acid phenethyl ester (CAPE) on chronicapplicationof tenofovir may arise result of nephrotoxicity, hepatotoxicity, neurotoxicity and pancreas damage. Methods: The study weighing 200-300 g adult Wistar albino rats was carried out on 21 female sex.The rats were randomized into control, tenofovir, CAPE, including tenofovir+CAPE were divided into 4 groups. T in chronic studies in rats for 30 days at 30 mg/kg/day oral doses was administered. Then CAPE intraperitoneally 10 micromol/kg dose was administered. Biochemical and histopathological examinations were performed. Findings: In histomorphological damage to the pancreas in group T, T+CAPE group, the damage is reduced and beta-cell density decreased in T group to the control group, T+CAPE group increased by only T was determined that the group. In kidney tissues; T and tubuler increasing apoptosis induced toxicity, but the use of T+CAPE was determined to prevent the tubuler toxicity. In brain, Inflammation in T group, vacuolar degeneration and nekrobiotik changes observed, however, a moderate improvement in T+CAPE group viewed. In the liver, where damageoccurs in T group, T+CAPE group is of the level of apoptosis compared with the group treated only T fell and regenerative changes wereobserved and focal cytoplasmic hypereosinophilia. Biochemically of not CAPE and raises T to reduce the levels of creatinine were determined in particular. Histopathological findings confirmed the biochemical findings. Result: Chronic use of all of the liver, brain, pancreas and kidney tissue damage caused, but after tenofovir use CAPE; can reduce the toxicity was determined. Accordingly, the purpose of CAPE on the use of prophylactic antiviral treatment should be made more long-term studies have concluded.
Purpose: To investigation of protective effects of caffeic acid phenethyl ester (CAPE) on chronicapplicationof tenofovir may arise result of nephrotoxicity, hepatotoxicity, neurotoxicity and pancreas damage. Methods: The study weighing 200-300 g adult Wistar albino rats was carried out on 21 female sex.The rats were randomized into control, tenofovir, CAPE, including tenofovir+CAPE were divided into 4 groups. T in chronic studies in rats for 30 days at 30 mg/kg/day oral doses was administered. Then CAPE intraperitoneally 10 micromol/kg dose was administered. Biochemical and histopathological examinations were performed. Findings: In histomorphological damage to the pancreas in group T, T+CAPE group, the damage is reduced and beta-cell density decreased in T group to the control group, T+CAPE group increased by only T was determined that the group. In kidney tissues; T and tubuler increasing apoptosis induced toxicity, but the use of T+CAPE was determined to prevent the tubuler toxicity. In brain, Inflammation in T group, vacuolar degeneration and nekrobiotik changes observed, however, a moderate improvement in T+CAPE group viewed. In the liver, where damageoccurs in T group, T+CAPE group is of the level of apoptosis compared with the group treated only T fell and regenerative changes wereobserved and focal cytoplasmic hypereosinophilia. Biochemically of not CAPE and raises T to reduce the levels of creatinine were determined in particular. Histopathological findings confirmed the biochemical findings. Result: Chronic use of all of the liver, brain, pancreas and kidney tissue damage caused, but after tenofovir use CAPE; can reduce the toxicity was determined. Accordingly, the purpose of CAPE on the use of prophylactic antiviral treatment should be made more long-term studies have concluded.
Açıklama
Anahtar Kelimeler
Gastroenteroloji, Gastroenterology
