Eryngium campestre ekstresinin deri kanseri üzerine etkilerinin araştırılması
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
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Cilt Başlığı
Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Apoptozisin kanserin gelişimi ve tedavisindeki önemi büyüktür. Çalışmamızın materyali olarak seçilen Eryngium campestre bitkisinden elde edilen bileşikler ile yapılmış az sayıda çalışma mevcut olmakla beraber, bu çalışmalar bitkinin bazı kanser türleri üzerinde aktif olabileceğini göstermiştir. Bu bilgilerden hareketle Eryngium campestre ekstresiyle muamele edilen cilt kanseri hücrelerinde kaspaz-3, Bax, Bcl-2, wee1, grp78, GADD153, ve AIF protein düzeyleri ve aktivitelerini incelemeyi amaçladık. Hücre kültürünün kullanıldığı çalışmada ELISA yöntemi ile kaspaz-3, Bax, Bcl-2, wee1, GADD153, AIF ve grp78 protein düzeyleri ve aktiviteleri incelendi. Çalışmamızda veriler %95 güvenle SPSS 21 versiyon kullanılarak analiz edildi. Grup kıyaslamaları için Mann Whitney U testi kullanıldı. Çalışma sonunda wee1, Bax, kaspaz-3, GADD153 ve AIF, grp78 protein düzeyleri ve aktiviteleri değerlendirildiğinde kontrole kıyasla anlamlı şekilde arttığı ve Bcl-2 değerlerinin azaldığı tespit edildi (p<0,01). Sonuç olarak; Eryngium campestre bitkisinden elde edilen bileşikler ile kanser tedavisinde kullanılma potansiyeli olabilecek yeni ilaçların geliştirilebileceği, bu amaçla in-vivo ve faz çalışmalarının yapılması gerektiği kanaatine varıldı.
Apoptosis is of great importance in the development and treatment of cancer. Few studies have been made with compounds from the Eryngium campestre plant which selected as the material of our study, these studies have shown that the plant may be active on some types of cancer. Based on this information, we aimed to examine caspase-3, Bax, Bcl-2, wee1, grp78, GADD153, and AIF protein levels and activities in skin cancer cells treated with Eryngium campestre extract. In the study, cell culture was used and caspase-3, Bax, Bcl-2, wee 1, GADD153, AIF and grp78 protein levels and activities were examined by ELISA method. In our study, data were analyzed using SPSS 21 version with 95% confidence. Mann Whitney U test was used for group comparisons. At the end of the study, wee 1, Bax, caspase-3, GADD153, AIF and grp78 protein levels and activities were evaluated and found to be significantly increased compared to control. Bcl-2 values were found to decrease (p<0,01). As a result; It was concluded that the compounds obtained from Eryngium campestre plant and new drugs that could potentially be used in cancer treatment could be developed and in-vivo and phase studies should be conducted for this purpose.
Apoptosis is of great importance in the development and treatment of cancer. Few studies have been made with compounds from the Eryngium campestre plant which selected as the material of our study, these studies have shown that the plant may be active on some types of cancer. Based on this information, we aimed to examine caspase-3, Bax, Bcl-2, wee1, grp78, GADD153, and AIF protein levels and activities in skin cancer cells treated with Eryngium campestre extract. In the study, cell culture was used and caspase-3, Bax, Bcl-2, wee 1, GADD153, AIF and grp78 protein levels and activities were examined by ELISA method. In our study, data were analyzed using SPSS 21 version with 95% confidence. Mann Whitney U test was used for group comparisons. At the end of the study, wee 1, Bax, caspase-3, GADD153, AIF and grp78 protein levels and activities were evaluated and found to be significantly increased compared to control. Bcl-2 values were found to decrease (p<0,01). As a result; It was concluded that the compounds obtained from Eryngium campestre plant and new drugs that could potentially be used in cancer treatment could be developed and in-vivo and phase studies should be conducted for this purpose.
Açıklama
Anahtar Kelimeler
Eczacılık ve Farmakoloji, Pharmacy and Pharmacology
