Detection of trichomonas vaginalis in vaginal speciemens from women by wet mount, culture and PCR
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Tarih
2015
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Trikomonosiosis, seksüel geçişli enfeksiyona sebep olan Trichomonas vaginalis nedenlidir. T.vaginalis, dünyada 180 milyon insanı etkilemektedir ve anlamlı morbidite sebebidir. T.vajinalis enfeksiyonu kadınlarda vajinit, ekzoservisit ve üretrit nedenidir. Bu çalışmanın amacı, üç değişik yöntem sonuçlarını karşılaştırarak T. vajinalis varlığını tespit etmektir. Gereç ve Yöntem: Mustafa Kemal Üniversitesi Tıp Fakültesi Jinekoloji polikliniğine ve Doğumevi hastanesine başvuran toplam 200 hastadan alınan swap örnekleri toplandı. : Mustafa Kemal Üniversitesi Tıp Fakültesi Parazitoloji bölümünde T. vajinalis genotip ve fenotipileri değerlendirildi. T. vaginalis tespiti açısından ve hasta tedavisine etkisi açısından bu çalışma bölgemizde yapılmış ilk moleküler çalışmadır. Bulgular: 200 hastanın 56 sı pozitif olarak bulundu ve bunların 24'ü (%42.8) mikroskopik olarak, 18'i (%32,1) kültür ile, 24'ü (%42,8) PCR ile tespit edildi. Tüm metodlarla tespit edilen hasta sayısı 14 idi. Bu çalışmada, Cochran's Q testi ile elde edilen üç metod karşılaştırılması anlamlı idi.(p=0.022). McNemar yöntemi ile tüm yöntemler ikili olarak karşılaştırıldığında mikroskopi ile kültür arasında (p=0.5),mikroskopi ile PCR arasında (p=0.063), kültür ve PCR arsında (p=0.25) fark bulunamadı. Tartışma: Kültür metodu rutinde kulanılan bir metod değildir ve kontaminasyon riski vardır. PCR metodu direkt olarak parazit DNA'sını göstermektedir ve diğer yöntemlere göre daha güvenilir bir yöntemdir.
Aim: Trichomoniasis, a sexually transmitted infection (STI) caused by Trichomonas vaginalis, affects 180 million people worldwide and causes significant morbidity. Infection with T. vaginalis has been associated with vaginitis, exocervicitis, and urethritis in women. Material and Method: In this study, we aim to investigate the presence of T. vaginalis by using three different methods for comparing the results. Two hundred T. vaginalis isolates taken from swap samples were collected in Medical Faculty, Department of Gynecology, Mustafa Kemal University Polyclinic, and examined genotypically and phenotypically to identify T. vaginalis in Parasitology Department. This research is unique in terms of its contribution to patient treatment, being the first molecular study in Turkey/Hatay to determine Trichomonas (TV) genes stemming from Trichomonas vaginalis strains. Result: 56 out of 200 patients examined were identified as positive and 24 (42.8%) of these were identified through microscopy, 18 (32,1%) with culture and 24 (42,8%) with PCR. The number of those identified through all these methods is 14 (25%). In this study, difference was calculated using three methods (p=0.022) with Cochran's Q test. When compared with McNemar two by two, no superiority in T. vaginalis diagnosis was found between microscopy and culture (p=0.5), microscopy and PCR (p=0.063), or culture and PCR (p=0.25) methods. Discussion: Culture method is not used in routine laboratory procedures and has contamination risk. PCR method shows directly the parasite of DNAs, and so it is thought to be more reliable compared to the other two methods.
Aim: Trichomoniasis, a sexually transmitted infection (STI) caused by Trichomonas vaginalis, affects 180 million people worldwide and causes significant morbidity. Infection with T. vaginalis has been associated with vaginitis, exocervicitis, and urethritis in women. Material and Method: In this study, we aim to investigate the presence of T. vaginalis by using three different methods for comparing the results. Two hundred T. vaginalis isolates taken from swap samples were collected in Medical Faculty, Department of Gynecology, Mustafa Kemal University Polyclinic, and examined genotypically and phenotypically to identify T. vaginalis in Parasitology Department. This research is unique in terms of its contribution to patient treatment, being the first molecular study in Turkey/Hatay to determine Trichomonas (TV) genes stemming from Trichomonas vaginalis strains. Result: 56 out of 200 patients examined were identified as positive and 24 (42.8%) of these were identified through microscopy, 18 (32,1%) with culture and 24 (42,8%) with PCR. The number of those identified through all these methods is 14 (25%). In this study, difference was calculated using three methods (p=0.022) with Cochran's Q test. When compared with McNemar two by two, no superiority in T. vaginalis diagnosis was found between microscopy and culture (p=0.5), microscopy and PCR (p=0.063), or culture and PCR (p=0.25) methods. Discussion: Culture method is not used in routine laboratory procedures and has contamination risk. PCR method shows directly the parasite of DNAs, and so it is thought to be more reliable compared to the other two methods.
Açıklama
Anahtar Kelimeler
Genel ve Dahili Tıp
Kaynak
Journal of Clinical and Analytical Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
6
Sayı
5
