In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide
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Date
2016
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Journal ISSN
Volume Title
Publisher
Springer
Access Rights
info:eu-repo/semantics/openAccess
Abstract
1-Naphthaleneacetamide (NAAm) is a synthetic plant growth regulator in the auxin family that is widely used in agriculture to promote the growth of numerous fruits, for root cuttings and as a fruit thinning agent. The potential genotoxic effects of NAAm were investigated in vitro by the chromosome aberrations (CAs), and cytokinesis-block micronucleus assays in human peripheral blood lymphocytes (PBLs) for the first time. The human PBLs were treated with 20, 40, 80, and 160 A mu g/mL of NAAm for 24 and 48 h. The results of this study showed that NAAm significantly induced the formation of structural CA and MN for all concentrations (20, 40, 80 and 160 A mu g/mL) and treatment periods (24 and 48 h) when compared with the negative and the solvent control. In addition, the higher concentrations of NAAm (80 and 160 A mu g/mL) caused a statistically significant increase in nuclear bud (NBUD) formation for both 24 and 48 h treatment times. With regard to the cell cycle kinetics, at all the tested concentrations, NAAm caused a statistically significant reduction in the mitotic index (MI) only for 48 h treatment period and also in the nuclear division index (NDI) for both 24 and 48 h treatment periods as compared to the control groups. The reductions in the MI and NDI occured in a concentration-dependent manner for both treatment times. In conclusion, the present results indicate that in the tested experimental conditions, NAAm was genotoxic and cytotoxic on human PBLs in vitro.
Description
Keywords
1-Naphthaleneacetamide, Synthetic plant growth regulator, Chromosome aberration, Micronucleus, Human peripheral blood lymphocytes
Journal or Series
Cytotechnology
WoS Q Value
Q3
Scopus Q Value
Q3
Volume
68
Issue
4
