Flowerlike hybrid horseradish peroxidase nanobiocatalyst for the polymerization of guaiacol
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, the catalytic activity and stability of flowerlike hybrid horseradish peroxidase (HRP) nanobiocatalyst (HRP-Cu2+) obtained from Cu2+ ions and HRP enzyme in the polymerization reaction of guaiacol were analyzed. We demonstrated that HRP-Cu2+ and hydrogen peroxide (H2O2) initiator showed significantly increased catalytic activity and stability on the polymerization of guaiacol compared to that of free HRP enzyme. Poly(guaiacol) was observed with quite high yields (88%) and molecular weights (38,000 g/mol) under p11 7.4 phosphate-buffered saline (PBS) conditions at 60 degrees C. with 5 weight% of HRP-Cu2+ loading. HRP-Cu2+ also shows very high thermal stability and works even at 70 degrees C reaction temperature; free HRP enzyme denatures at that temperature. Furthermore, HRP-Cu2+ provided considerable repeated use and showed some degree of catalytic activity, even after the fourth recycle, in the polymerization of guaiacol.
Açıklama
Anahtar Kelimeler
Horseradish peroxidase, hybrid nanoflowers, polymerization, guaiacol
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
44
Sayı
5
