Isolation and localization of new germination-related sequences from wheat embryos
Yükleniyor...
Dosyalar
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Singapore Pte Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Subtractive library hybridization was used to isolate the cDNA clones that corresponded to the transcripts that were specifically up-regulated during wheat embryo germination. The clones with numbers 5, 6, 7, 8, 24, and 26 appeared to be more abundant in germinating wheat embryos. Among the isolated clones, we identified four new members of the wheat germin gene family. We also identified two novel sequences which exhibited distinct germination up-regulation, and displayed characteristic spatial patterns of expression. One of these, represented by clone pSB10, was principally expressed in the root tissue of germinating embryos. The second was represented by the pSB7 clone and was expressed in both the root and shoot primordia of the embryonic axis, as well as within the coleoptile.
Açıklama
Anahtar Kelimeler
germin, germination, in situ RNA hybridization, subtractive hybridization
Kaynak
Journal of Biochemistry and Molecular Biology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
36
Sayı
6
