Isolation and localization of new germination-related sequences from wheat embryos

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dc.contributor.authorCaliskan, M
dc.contributor.authorBashiardes, S
dc.contributor.authorOzcan, B
dc.contributor.authorCuming, AC
dc.date.accessioned2024-09-18T20:25:21Z
dc.date.available2024-09-18T20:25:21Z
dc.date.issued2003
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractSubtractive library hybridization was used to isolate the cDNA clones that corresponded to the transcripts that were specifically up-regulated during wheat embryo germination. The clones with numbers 5, 6, 7, 8, 24, and 26 appeared to be more abundant in germinating wheat embryos. Among the isolated clones, we identified four new members of the wheat germin gene family. We also identified two novel sequences which exhibited distinct germination up-regulation, and displayed characteristic spatial patterns of expression. One of these, represented by clone pSB10, was principally expressed in the root tissue of germinating embryos. The second was represented by the pSB7 clone and was expressed in both the root and shoot primordia of the embryonic axis, as well as within the coleoptile.en_US
dc.identifier.endpage585en_US
dc.identifier.issn1225-8687
dc.identifier.issue6en_US
dc.identifier.pmid14659077en_US
dc.identifier.scopus2-s2.0-0942278890en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage580en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10260
dc.identifier.volume36en_US
dc.identifier.wosWOS:000186763400010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Singapore Pte Ltden_US
dc.relation.ispartofJournal of Biochemistry and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectgerminen_US
dc.subjectgerminationen_US
dc.subjectin situ RNA hybridizationen_US
dc.subjectsubtractive hybridizationen_US
dc.titleIsolation and localization of new germination-related sequences from wheat embryosen_US
dc.typeArticleen_US

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