Differentiation of some myxomycetes species by ITS sequences

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dc.contributor.authorBaba, Hayri
dc.contributor.authorKolukırık, Mustafa
dc.contributor.authorZümre, Murat
dc.date.accessioned2019-07-16T16:00:28Z
dc.date.available2019-07-16T16:00:28Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractSeven different PCR primers targeting 18 different myxomycetes genera were designed and ribosomal DNA ITS1-5.8SrDNAITS2 regions of 52 morphologically characterized species from Turkey were amplified and sequenced. The studied species belong to the genera Arcyria, Badhamia, Ceratiomyxa, Collaria, Comatricha, Cribraria, Diachea, Diderma, Didymium, Enerthenema, Lamproderma, Lycogala, Perichaena, Physarum, Reticularia, Stemonitis, Stemonitopsis, and Trichia. Significant ITS region size and sequence variations were observed among the analyzed species. The ITS based phylogenetic tree did not reflect evolutionary relationships at genus or higher level. The only exception was the genus Didymium, which formed a clearly distinctive cluster in the phylogenetic tree containing 18 different genera. It was concluded that interspecies variation of the ITS1-5.8S-ITS2 region is high enough to differentiate species, but this region did not reflect the evolutionary relationships between the myxomycetes species. Hence, we suggested that sequencing of the ITS region could only be used as a supporting tool for the phenotypic identification, not for revealing the evolutionary relationships between the myxomycetes species.en_US
dc.description.abstractSeven different PCR primers targeting 18 different myxomycetes genera were designed and ribosomal DNA ITS1-5.8SrDNAITS2 regions of 52 morphologically characterized species from Turkey were amplified and sequenced. The studied species belong to the genera Arcyria, Badhamia, Ceratiomyxa, Collaria, Comatricha, Cribraria, Diachea, Diderma, Didymium, Enerthenema, Lamproderma, Lycogala, Perichaena, Physarum, Reticularia, Stemonitis, Stemonitopsis, and Trichia. Significant ITS region size and sequence variations were observed among the analyzed species. The ITS based phylogenetic tree did not reflect evolutionary relationships at genus or higher level. The only exception was the genus Didymium, which formed a clearly distinctive cluster in the phylogenetic tree containing 18 different genera. It was concluded that interspecies variation of the ITS1-5.8S-ITS2 region is high enough to differentiate species, but this region did not reflect the evolutionary relationships between the myxomycetes species. Hence, we suggested that sequencing of the ITS region could only be used as a supporting tool for the phenotypic identification, not for revealing the evolutionary relationships between the myxomycetes species.en_US
dc.identifier.endpage382en_US
dc.identifier.issn1300-008x
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84926333218en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage377en_US
dc.identifier.urihttps://trdizin.gov.tr/publication/paper/detail/TWpFek9UQXhNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12483/2341
dc.identifier.volume39en_US
dc.identifier.wosWOS:000352486200015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Botanyen_US
dc.relation.publicationcategoryDiğeren_US]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiyolojien_US
dc.titleDifferentiation of some myxomycetes species by ITS sequencesen_US
dc.typeOtheren_US

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