Vitrification of Common Carp (Cyprinus Carpio) Spermatozoa, Post-Thaw Sperm Quality, and Fertility
[ N/A ]
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Israeli Journal Of Aquaculture-Bamidgeh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this investigation was to test a new technology, vitrification, or ultra-rapid freezing of the spermatozoa of common carp, and to study the ability of glucose, BSA, and other cryoprotectants to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified using 10 different cryoprotectant solutions: (1) Glucose based medium (GBM) + 1% bovine serum albumin (BSA); (2) GBM + 1% BSA + 10% DMSO; (3) GBM + 1% BSA + 20% DMSO; (4) GBM + 1% BSA + 30% DMSO; (5) GBM + 1% BSA + 10% DMA; (6) GBM + 1% BSA + 20% DMA; (7) GBM + 1% BSA + 30% DMA; (8) GBM + 1% BSA + 10% methanol; (9) GBM + 1% BSA + 20% methanol; (10) GBM + 1% BSA + 30% methanol. Fertilization rates for vitrification experiments were low and the use of low concentrations of cryoprotectants yielded lower fertilization rates than the vitrification solutions containing high cryoprotectant concentrations. In conclusion, this study reported successful vitrification of common carp spermatozoa by direct transfer into liquid nitrogen.
Açıklama
Anahtar Kelimeler
vitrification, cryoprotectants, sperm quality, fertilization, Cyprinus carpio
Kaynak
Israeli Journal of Aquaculture-Bamidgeh
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
66
