Vitrification of Common Carp (Cyprinus Carpio) Spermatozoa, Post-Thaw Sperm Quality, and Fertility

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYildiz, Cengiz
dc.contributor.authorYavas, Ilker
dc.date.accessioned2024-09-18T21:00:37Z
dc.date.available2024-09-18T21:00:37Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of this investigation was to test a new technology, vitrification, or ultra-rapid freezing of the spermatozoa of common carp, and to study the ability of glucose, BSA, and other cryoprotectants to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified using 10 different cryoprotectant solutions: (1) Glucose based medium (GBM) + 1% bovine serum albumin (BSA); (2) GBM + 1% BSA + 10% DMSO; (3) GBM + 1% BSA + 20% DMSO; (4) GBM + 1% BSA + 30% DMSO; (5) GBM + 1% BSA + 10% DMA; (6) GBM + 1% BSA + 20% DMA; (7) GBM + 1% BSA + 30% DMA; (8) GBM + 1% BSA + 10% methanol; (9) GBM + 1% BSA + 20% methanol; (10) GBM + 1% BSA + 30% methanol. Fertilization rates for vitrification experiments were low and the use of low concentrations of cryoprotectants yielded lower fertilization rates than the vitrification solutions containing high cryoprotectant concentrations. In conclusion, this study reported successful vitrification of common carp spermatozoa by direct transfer into liquid nitrogen.en_US
dc.description.sponsorshipMustafa Kemal University Scientific Research Fund [M.K.U-08-E-0203]en_US
dc.description.sponsorshipThis study was partly supported by a grant from Mustafa Kemal University Scientific Research Fund (M.K.U-08-E-0203). The authors would like to thank the staff of the State Hydraulic Works (SHW) Fish Production Station in Adana (Turkey) for their technical assistance.en_US
dc.identifier.issn0792-156X
dc.identifier.scopus2-s2.0-84899147680en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12788
dc.identifier.volume66en_US
dc.identifier.wosWOS:000335918400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIsraeli Journal Of Aquaculture-Bamidgehen_US
dc.relation.ispartofIsraeli Journal of Aquaculture-Bamidgehen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectvitrificationen_US
dc.subjectcryoprotectantsen_US
dc.subjectsperm qualityen_US
dc.subjectfertilizationen_US
dc.subjectCyprinus carpioen_US
dc.titleVitrification of Common Carp (Cyprinus Carpio) Spermatozoa, Post-Thaw Sperm Quality, and Fertilityen_US
dc.typeArticleen_US

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