Cyclotide-rich fractions containing nanofibers by electrospinning: preparation, characterization and examination of antimicrobial activity

Basit Öğe Kaydı
dc.authoridAKCAN, Muharrem/0000-0002-5208-9575
dc.contributor.authorBas, Elif Busra
dc.contributor.authorKurekc, Cemil
dc.contributor.authorKalfa, Orhan Murat
dc.contributor.authorAkcan, Muharrem
dc.date.accessioned2024-09-18T20:14:58Z
dc.date.available2024-09-18T20:14:58Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this study, antimicrobial nanofibers were produced with the mixtures of polyvinyl alcohol (PVA) and cyclotide-rich fractions by electrospinning method. After extraction, the first separation was carried out with C18 flash chromatography and then fractioned into five separate parts by reversed-phase high-pressure liquid chromatography (RP-HPLC). The molecular weights of cyclotides in each fraction were determined by quadrupole time-of-flight liquid chromatography-mass spectrometry (Q-TOF LC-MS). Cyclotide-rich fractions were mixed with 10% of PVA solution and nanofibers were produced from this biocomposite mixture by electrospinning method. The nanofibers were characterized by field emission scanning electron microscopy (FE-SEM), and it was observed that 100% peptide-containing nanofibers (cyclotide-rich fraction/10% PVA, w/v) had more regular fiber textures. The presence of the peptides in the nanofiber was also confirmed by analytical RP-HPLC, as the peptides in both peptide fractions and nanofiber solutions have the same retention times. The nanofibers produced with the fourth cyclotide-rich fraction showed activity against gram-positive bacteria (Bacillus cereus) in antimicrobial susceptibility test. As a result of these findings, cyclotide-containing nanofibers with antimicrobial activity can be produced for pharmaceutical research and development studies.en_US
dc.identifier.doi10.55730/1300-0527.3468
dc.identifier.endpage1660en_US
dc.identifier.issn1300-0527
dc.identifier.issue5en_US
dc.identifier.pmid37529722en_US
dc.identifier.scopus2-s2.0-85143162641en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1651en_US
dc.identifier.trdizinid1142251en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0527.3468
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1142251
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9338
dc.identifier.volume46en_US
dc.identifier.wosWOS:000876350600026en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCyclotidesen_US
dc.subjectelectrospinningen_US
dc.subjectnanofibersen_US
dc.subjectantimicrobial activityen_US
dc.subjectdrug deliveryen_US
dc.titleCyclotide-rich fractions containing nanofibers by electrospinning: preparation, characterization and examination of antimicrobial activityen_US
dc.typeArticleen_US

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