IS METHOTREXATE-INDUCED TESTICULAR DAMAGE PREVENTABLE USING NETTLE SEED EXTRACT: A HISTOPATHOLOGICAL, IMMUNOHISTOCHEMICAL, BIOCHEMICAL AND SPERMATOLOGICAL EXAMINATION
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the study 32 Wistar albino rats were divided into four group as control (Group Control, n=8), methotrexate (Group MTX, n=8), MTX + UDS (Group MTX+UDS, n=8) and Urtica dioica seed extract (UDS) (Group UDS, n=8). After the trial, blood and post-necropsy testicular tissue samples were taken. Histopathological examinations showed that methotrexate had an adverse impact on spermatogenesis by damaging testicles; however, such damages were substantially decreased in the Group MTX+UDS. In the immunohistochemical examinations glutathione peroxidase 1 (GPx1) immunoreactive areas was higher in the Group MTX + UDS compared to the Group MTX. Biochemical examinations revealed that the level of malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST) and catalase (CAT) enzymes levels statistically significantly differenced (p<0.001) in the Group MTX compared to the control, UDS and MTX+UDS groups. There were significant (p<0.05) differences the Group MTX from Group MTX+UDS. such as density, motility, dead-live sperm rate and abnormal sperm rate. Our study results showed that UDS prevented the damage occurred in the testicles according to histopathological, immunohistochemical, biochemical and spermatological findings.
Açıklama
Anahtar Kelimeler
Methotrexate, Urtica dioica seed extract, Pathology, Testicles
Kaynak
Fresenius Environmental Bulletin
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
Cilt
27
Sayı
4
