Over, böbrek ve beyinde iskemi / reperfüzyon sonrası redoks dengesi ve Tribulus terrestris L.’nin etkileri
Yükleniyor...
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Attribution-NonCommercial 3.0 United States
Attribution-NonCommercial 3.0 United States
Özet
Amaç: Oksidatif stres prooksidan ve antioksidan sistemler arasında oksidasyon lehine bir dengesizlik olarak
tanımlanır ve iskemi / reperfüzyon hasarı gibi çeşitli patolojilerden sorumlu tutulmaktadır. Eksojen antioksidanlar
redoks dengesinin geri kazanılmasında faydalıdırlar. Tribulusterrestris L. (demir dikeni) güçlü antioksidan etki
sergileyen flavonoidler ve saponinler açısından zengin bir tıbbi bitkidir. Bu çalışma over, böbrek ve beyinde
reperfüzyon hasarında Tribulus ekstresinin redoks dengesi üzerine etkilerini araştırmayı hedeflemiştir.
Yöntemler: Dişi Wistar albino sıçanlaraTribulus veya fizyolojik salin uygulandı ve hayvanlar 60 dakika süreli over,
böbrek ya da beyin iskemisini takip eden 60 dakika süreli reperfüzyona maruz bırakıldılar. Bahsedilen dokularda total
antioksidan durum (TOS), total oksidan durum (TAS) ve oksidatif stres indeksi (OSI) belirlendi.
Bulgular: Tribulus’un over, böbrek ve beyinde yalnızca reperfüzyon hasarı oluşturulan hayvanlara kıyasla TOS’u
düşürdüğü bulundu (sırasıyla, p<0.001, p=0.004 ve p=0.035). Aynı zamanda Tribulus uygulamasıyla her üç dokuda
yalnızca reperfüzyon hasarı oluşturulan hayvanlara kıyasla redoks dengesinin önemli bir göstergesi olan OSI’de
azalma sağlandı (over: p<0.001, böbrek: p=0.007 ve beyin: p=0.002).
Sonuç: Bu bulgular Tribulus’un reperfüzyon hasarının yüksek oksidatif ortamında dahi güçlü bir antioksidan etki
sergilediğini ortaya koymaktadır. Oksidatif stresin reperfüzyon hasarının yıkıcı sürecine iştiraki düşünüldüğünde
Tribulus’un stresi sınırlayıcı etkisi, redoks dengesinin korunmasına katkı sunmak marifetiyle reperfüzyon hasarına
karşı faydalı olabileceğini göstermektedir.
Objective: Oxidative stress is defined as the imbalance in pro-oxidative and anti-oxidative systems in favor of oxidation, and it is held responsible for numerous pathologies including ischemia/reperfusion injury. Exogenous antioxidants are of benefit to the restoration of the redox balance. Tribulusterrestris L. (devil’s weed) is a medicinal herb, rich in flavonoids and saponins that exert strong anti-oxidant actions. The present study was aimed to investigate the effects of Tribulus extract on the redox balance in ovarian, renal and cerebral reperfusion injury. Method: Female Wistar albino rats were either received Tribulus or physiological saline and undergone to ovarian, renal or cerebral ischemia for 60 minutes followed by a 60-minute-long reperfusion. Total anti-oxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were estimated in the mentioned tissues. Results: Tribulus was found to reduce TOS in ovaries, kidneys and brain as compared with the animals in which only reperfusion injury was generated (respectively, p<0.001, p=0.004 and p=0.035). Meanwhile, a decrease in OSI, a pivotal indicator of the redox balance, was acquired with Tribulus administration in all three tissues compared with the animals in which only reperfusion injury was generated (ovary: p<0.001, kidney: p=0.007 and brain: p=0.002). Conclusion: These findings demonstrate that Tribulus possesses a strong anti-oxidant effect even in highly oxidative environment of reperfusion injury. Since oxidative stress participates in the destructive process in reperfusion injury, the stress-limiting feature of Tribulus can offer a use against the reperfusion injury through supporting maintenance of the redox balance.
Objective: Oxidative stress is defined as the imbalance in pro-oxidative and anti-oxidative systems in favor of oxidation, and it is held responsible for numerous pathologies including ischemia/reperfusion injury. Exogenous antioxidants are of benefit to the restoration of the redox balance. Tribulusterrestris L. (devil’s weed) is a medicinal herb, rich in flavonoids and saponins that exert strong anti-oxidant actions. The present study was aimed to investigate the effects of Tribulus extract on the redox balance in ovarian, renal and cerebral reperfusion injury. Method: Female Wistar albino rats were either received Tribulus or physiological saline and undergone to ovarian, renal or cerebral ischemia for 60 minutes followed by a 60-minute-long reperfusion. Total anti-oxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were estimated in the mentioned tissues. Results: Tribulus was found to reduce TOS in ovaries, kidneys and brain as compared with the animals in which only reperfusion injury was generated (respectively, p<0.001, p=0.004 and p=0.035). Meanwhile, a decrease in OSI, a pivotal indicator of the redox balance, was acquired with Tribulus administration in all three tissues compared with the animals in which only reperfusion injury was generated (ovary: p<0.001, kidney: p=0.007 and brain: p=0.002). Conclusion: These findings demonstrate that Tribulus possesses a strong anti-oxidant effect even in highly oxidative environment of reperfusion injury. Since oxidative stress participates in the destructive process in reperfusion injury, the stress-limiting feature of Tribulus can offer a use against the reperfusion injury through supporting maintenance of the redox balance.
Açıklama
Anahtar Kelimeler
Tribulus, Demir dikeni, Oksidatif stres, İskemi, Reperfüzyon, Tribulus, Devil’s weed, Oxidative stress, Ischemia, Reperfusion
Kaynak
Dicle Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
46
Sayı
3
Künye
Demir, E. A. (2019). Over, Böbrek ve Beyinde İskemi/Reperfüzyon Sonrası Redoks Dengesi ve Tribulus Terrestris L.’nin Etkileri. Dicle Tıp Dergisi, 46(3), 525-534.
