Identification of microorganism species in leafy vegetables using maldi-tof ms (Matrix assisted laser desorption / ionization mass spectrometry) technique
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Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Centenary University
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The foodborne fungal and bacterial pathogens are increasingly associated with fresh fruit and vegetables. Bacterial colonization of fresh produce plants can occur to high levels on the external tissue but bacteria are detected within plant tissue. Leafy vegetables such as lettuce, spinach, cabbage, rocket, mint, parsley, dill, and leek were collected from different vegetable markets in the counties of Defne and Antakya of Hatay province. Growing media potato dextrose agar, nutrient agar and blood agar media were used for microbiological analysis. Their identification were performed based on phenotypic characters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique. According to the results of MALDI-TOF MS analysis, when the distribution of microorganisms was examined, the most common bacterial species were identified with 60 species at the species level, followed by 4 fungi and 1 yeast. The most common microorganism species were obtained from lettuce samples. When the distribution of species level is examined; firstly, 9 species with Pseudomonas, 5 species with Lactobacillus, 4 species with Acinetobacter and 2 species with Klebsiella, Citrobacter, Enterobacter, Lysinibacillus were obtained and Pantoea one species Bacillus, one species Escherichia coli, one species followed by Exiguobacterium genera. When the colony density of microorganism species was examined, the most intensive colony was determined in the species belonging to the genus Pseudomonas and Acinetobacter. At least colony density was observed in fungi. In result, Salmonella sp, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Klebsiella oxytoca species were found to be very dangerous species for human health. © 2019, Centenary University. All rights reserved.
Açıklama
Anahtar Kelimeler
Fresh vegetables, Identification, MALDI-TOF MS, Microorganism
Kaynak
Yuzuncu Yil University Journal of Agricultural Sciences
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
29
Sayı
4
