Identification of microorganism species in leafy vegetables using maldi-tof ms (Matrix assisted laser desorption / ionization mass spectrometry) technique

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dc.authorscopusid55780588400
dc.authorscopusid6601978020
dc.authorscopusid7004656210
dc.authorscopusid6603505625
dc.authorscopusid57194387076
dc.contributor.authorUysal, Aysun
dc.contributor.authorKurt, Şener
dc.contributor.authorSoylu, Soner
dc.contributor.authorSoylu, E. Mine
dc.contributor.authorKara, Merve
dc.date.accessioned2024-09-19T15:48:30Z
dc.date.available2024-09-19T15:48:30Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe foodborne fungal and bacterial pathogens are increasingly associated with fresh fruit and vegetables. Bacterial colonization of fresh produce plants can occur to high levels on the external tissue but bacteria are detected within plant tissue. Leafy vegetables such as lettuce, spinach, cabbage, rocket, mint, parsley, dill, and leek were collected from different vegetable markets in the counties of Defne and Antakya of Hatay province. Growing media potato dextrose agar, nutrient agar and blood agar media were used for microbiological analysis. Their identification were performed based on phenotypic characters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique. According to the results of MALDI-TOF MS analysis, when the distribution of microorganisms was examined, the most common bacterial species were identified with 60 species at the species level, followed by 4 fungi and 1 yeast. The most common microorganism species were obtained from lettuce samples. When the distribution of species level is examined; firstly, 9 species with Pseudomonas, 5 species with Lactobacillus, 4 species with Acinetobacter and 2 species with Klebsiella, Citrobacter, Enterobacter, Lysinibacillus were obtained and Pantoea one species Bacillus, one species Escherichia coli, one species followed by Exiguobacterium genera. When the colony density of microorganism species was examined, the most intensive colony was determined in the species belonging to the genus Pseudomonas and Acinetobacter. At least colony density was observed in fungi. In result, Salmonella sp, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Klebsiella oxytoca species were found to be very dangerous species for human health. © 2019, Centenary University. All rights reserved.en_US
dc.identifier.doi10.29133/yyutbd.627850
dc.identifier.endpage603en_US
dc.identifier.issn1308-7576
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85077616737en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage595en_US
dc.identifier.urihttps://doi.org/10.29133/yyutbd.627850
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15092
dc.identifier.volume29en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherCentenary Universityen_US
dc.relation.ispartofYuzuncu Yil University Journal of Agricultural Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFresh vegetablesen_US
dc.subjectIdentificationen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectMicroorganismen_US
dc.titleIdentification of microorganism species in leafy vegetables using maldi-tof ms (Matrix assisted laser desorption / ionization mass spectrometry) techniqueen_US
dc.title.alternativeYaprağı yenen sebzelerdeki mikroorganizma türlerinin maldi-tof ms (Matris destekli lazer desorpsiyon/iyonizasyon uçuş süresi kütle spektrometresi) tekniği kullanılarak tanılanmasıen_US
dc.typeArticleen_US

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