Isolation and Identification of Bacterial Soft Rot Disease Agent Pectobacterium Isolates on Potato Tubers Delivered for Consumption in Yozgat and Kirsehir Provinces

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dc.authoridSOYLU, Soner/0000-0003-1002-8958
dc.authoridSoylu, Emine Mine/0000-0001-5961-0848
dc.contributor.authorOzturk, Murat
dc.contributor.authorSoylu, Soner
dc.date.accessioned2024-09-18T20:54:07Z
dc.date.available2024-09-18T20:54:07Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPectobacterium spp. are ubiquitous bacterial plant pathogens with a wide host range which cause soft rot disease on the invaded plant tissue. This study aimed to isolate the bacterial soft rot disease agents belonging to Pectobacterium spp. on potato tubers delivered for consumption in the local bazaar, grocery and markets in the Center district of Yozgat province and Kaman district of Kirsehir province of Turkey between 2018 and 2019. Local bazaar, market and grocery stores were surveyed and 168 potato tubers were collected. The humidity cell test was performed to investigate the presence of pectinolytic bacteria in asymptomatic and soft rotted potato tubers. Bacterial isolation from 54 different soft rotted tuber slices was performed on Crystal Violet Pectate (CVP) medium. A total of 42 bacterial isolates, that formed pits on CVP medium, were obtained following isolation. Among these, 27 isolates were observed to have shiny, transparent, irregular, crater-shaped colony morphology on Nutrient Agar (NA) plates. All isolates were gram and oxidase negative, catalase-positive, facultative anaerobe, non-fluorescent morphological characteristics and caused soft rot on potato tuber slices. Following PCR analysis, 19 of 54 symptomatic tuber samples were determined as infected by P. carotovorum subsp. carotovorum (9 isolates), P. atrosepticum (6 isolates) and Pectobacterium spp. (4 isolates). In 8 of 114 asymptomatic tuber samples, P. carotovorum subsp. carotovorum (5 isolates), P. atrosepticum (2 isolates) and P. carotovorum subsp. brasiliense (1 isolate) were determined as responsible agents of latent infections. The presence of Pectobacterium isolates in the stored potato tubers brought for consumption suggests the pathogen can be transferred to different regions. Potato tubers offered for sale for consumption are thought to be an important inoculum source for the transfer of Pectobacterium isolates to disease-free areas.en_US
dc.identifier.doi10.33462/jotaf.974350
dc.identifier.endpage342en_US
dc.identifier.issn1302-7050
dc.identifier.issn2146-5894
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85131632367en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage332en_US
dc.identifier.trdizinid1152638en_US
dc.identifier.urihttps://doi.org/10.33462/jotaf.974350
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1152638
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11605
dc.identifier.volume19en_US
dc.identifier.wosWOS:000813467900010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isotren_US
dc.publisherUniv Namik Kemalen_US
dc.relation.ispartofJournal of Tekirdag Agriculture Faculty-Tekirdag Ziraat Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPectobacterium spp.en_US
dc.subjectSoft roten_US
dc.subjectLatently infected tuberen_US
dc.subjectPotatoen_US
dc.subjectPCRen_US
dc.titleIsolation and Identification of Bacterial Soft Rot Disease Agent Pectobacterium Isolates on Potato Tubers Delivered for Consumption in Yozgat and Kirsehir Provincesen_US
dc.typeArticleen_US

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