Preeklampsi de dinamik tiyol
Yükleniyor...
Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Plasentadaki anormalliklere bağlı olarak gelişen preeklampsi, gebeliğin ikinci yarısından itibaren ortaya çıkar. Endoteliyal disfonksiyona bağlı olarak gelişen hipertansiyon ile karakterizedir. Buna bağlı olarakta maternal ve perinatal mortaliteye sebep olur. Preeklampsi patogenezinde vasküler problemlerin yanında oksidatif dengenin bozulması ve inflamasyon artışıda önemli etkenlerdir. Biz bu çalışmada preeklampsi gelişen gebelerde plazma inflamatuvar sitokin TNF-α ile sistemik tiyol gruplarının düzeyini ölçerek, normal gebelerle karşılaştırmayı hedefledik. Bu amaçla 30 Preeklampsili gebe hasta ve 30 sağlıklı gebe kontrol grubu olarak çalışmaya alındı. Tüm gebelerden 2. veya 3. trimesterde sabah açlık kanları toplanarak – 80 °C saklandı. Serum örneklerinde tiyol düzeyleri otoanalizörde, TNF-α ise ELİSA yöntemi ile ölçülmüştür. Veriler istatistiksel olarak analiz edildi ve tartışıldı. Sonuçta preeklampsi grubunda disülfit ve TNF-α düzeyleri kontrollere göreanlamlı derecede yüksek iken Nativ tiyoldüzeyleri anlamlı dercede düşük bulundu (P<0,001). Demografik bilgilerdeki sonuçlarda ise çalışma ve konrol grubları arasında gebelik haftası ile doğum ağırlığı anlamlı derecede düşükken, SistolikTA ve diastolikTA kan basınçları ise anlamlı derecede yüksek bulundu (P<0,001). Kontrol grubu ile preeklampsi grubu arasında yaş bakımından anlamlı fark bulunamadı. Sonuç olarak preeklampsi hasta grubunda serum disülfit miktarı ve inflamasyon artmıştır.
Preeclampsia due to abnormalities in the placenta occurs as of the second half of pregnancy. It is characterized by hypertension due to endothelial dysfunction. Therefore, it causes maternal and perinatal mortality. In the pathogenesis of preeclampsia, besides vascular problems, disruption of oxidative balance and increase in inflammation are important factors. In this study, we aimed to compare plasma inflammatory cytokine TNF-α and systemic thiol groups in pregnant women with preeclampsia and compare with normal pregnant women. For this purpose, 30 pregnant women with preeclampsia and 30 healthy pregnant women were included in the study. Fasting blood was collected from all pregnant women in the 2nd or 3rd trimester and stored at - 80 ° C. Thiol levels in serum samples were measured by autoanalyser and TNF-α was measured by ELISA. The data were analyzed statistically and discussed. As a result, disulfide and TNF-α levels were significantly higher in the preeclampsia group compared to controls, while the native thiol levels were significantly lower (P <0.001). Demographic data showed that gestational age and birth weight were significantly lower, and systolicTA and diastolicTA blood pressures were significantly higher between the study and control groups (P <0.001). No significant difference was found between the control group and preeclampsia group in terms of age. As a result, serum disulfide content and inflammation increased in preeclampsia patients.
Preeclampsia due to abnormalities in the placenta occurs as of the second half of pregnancy. It is characterized by hypertension due to endothelial dysfunction. Therefore, it causes maternal and perinatal mortality. In the pathogenesis of preeclampsia, besides vascular problems, disruption of oxidative balance and increase in inflammation are important factors. In this study, we aimed to compare plasma inflammatory cytokine TNF-α and systemic thiol groups in pregnant women with preeclampsia and compare with normal pregnant women. For this purpose, 30 pregnant women with preeclampsia and 30 healthy pregnant women were included in the study. Fasting blood was collected from all pregnant women in the 2nd or 3rd trimester and stored at - 80 ° C. Thiol levels in serum samples were measured by autoanalyser and TNF-α was measured by ELISA. The data were analyzed statistically and discussed. As a result, disulfide and TNF-α levels were significantly higher in the preeclampsia group compared to controls, while the native thiol levels were significantly lower (P <0.001). Demographic data showed that gestational age and birth weight were significantly lower, and systolicTA and diastolicTA blood pressures were significantly higher between the study and control groups (P <0.001). No significant difference was found between the control group and preeclampsia group in terms of age. As a result, serum disulfide content and inflammation increased in preeclampsia patients.
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry ; Kadın Hastalıkları ve Doğum
