Flow cytometric analysis of lymphocyte subsets, monocytes, and HLA-DR expressions on these cells in patients with COVID-19

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dc.authoridYalcindag, Ali/0000-0003-1846-9248
dc.authoridozcan, oguzhan/0000-0001-7486-503X
dc.contributor.authorOzcan, Nurgul
dc.contributor.authorCaglayan, Murat
dc.contributor.authorYalcindag, Ali
dc.contributor.authorOzcan, Oguzhan
dc.date.accessioned2024-09-18T20:13:27Z
dc.date.available2024-09-18T20:13:27Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjectives We aimed to investigate the lymphocyte subsets and monocytes by flow cytometry and the correlations between their HLA-DR expressions and inflammatory markers in patients with COVID-19. Methods The study included 49 patients with COVID-19 and 42 healthy controls. Blood samples were taken into EDTA tubes. WBC counts were analyzed by the Sysmex/XN-1000i device, and lymphocyte subsets and monocytes were analyzed by flow cytometry. The percentage of HLA-DR expression on cells and median fluorescence intensity (MFI) values were recorded to detect activation. Lymphocyte counts were calculated using the dual-platform method. Correlations between antigen expression and ferritin, CRP, and D-dimer levels were analyzed. Results The patient group had lower WBC and lymphocyte counts but significantly higher monocyte counts and neutrophil/lymphocyte ratios compared to controls (p=0.009, p=0.045, respectively). The patient group had significantly lower T lymphocyte counts (p=0.008). B lymphocyte counts and percentages were lower (p<0.001, p=0.004) in the patient group. There was no significant difference between the two groups in terms of NK cells. T helper and T cytotoxic lymphocyte counts were significantly lower, but there was no change in CD4/CD8 ratios. The percentage of HLA-DR expression on T lymphocytes, HLA-DR MFI values of T cytotoxic cells, and HLA-DR MFI values of CD16+ monocytes were significantly increased in the patient group (p=0.001, p=0.004, p<0.001, respectively). CRP was positively correlated with HLA-DR expression on T lymphocytes (r=0.501, p<0.001). Conclusions HLA-DR MFI values may be an important marker for demonstrating the function of both T cytotoxic cells and CD16+ monocytes in COVID-19.en_US
dc.description.sponsorshipBeckman Coulter Biyomedikal rnler San. ve Tic. Ltd. Scedil;ti.en_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1515/tjb-2023-0096
dc.identifier.endpage196en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85193278931en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage187en_US
dc.identifier.trdizinid1243697en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2023-0096
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1243697
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9196
dc.identifier.volume49en_US
dc.identifier.wosWOS:001161921200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCOVID-19en_US
dc.subjectlymphocyte subsetsen_US
dc.subjectmonocytesen_US
dc.subjectHLA-DRen_US
dc.subjectCD16en_US
dc.titleFlow cytometric analysis of lymphocyte subsets, monocytes, and HLA-DR expressions on these cells in patients with COVID-19en_US
dc.typeArticleen_US

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