Transferability of SSR markers from distantly related legumes to Glycyrrhiza species
Yükleniyor...
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Licorice (Glycyrrhiza spp.) is an important medicinal plant and its distribution of natural habitats has been shrinking day by day due to extensive collection. Genetic diversity identifcation will likely assist in conserving the diferent Glycyrrhiza species. A total of 127 simple sequence repeat (SSR) markers from Medicago truncatula Gaertn., Phaseolus vulgaris L., and Cicer arietinum L. were used and 26 of them were amplifed on Glycyrrhiza genomes. Te highest transferability rate (33%) was obtained from M. truncatula markers, while the highest genetic diversity values were obtained in P. vulgaris markers. Te markers BM153 and PV-ag004 from P. vulgaris had the largest polymorphism information content. Te amplifed primers were also used to identify genetic diversity among three Glycyrrhiza species. Te gene diversity values among Glycyrrhiza species appeared to be similar; however, donor species mostly had lower diversity values than those of Glycyrrhiza species. Additionally, the genetic analysis showed that G. favescens Boiss. subsp. favescens is more distantly related to species G. glabra L. var. glandulifera and G. echinata L. Te number of alleles was mostly higher than in the donor species, possibly proposing a multiallelic and/or polyploid structure in Glycyrrhiza species.
Licorice (Glycyrrhiza spp.) is an important medicinal plant and its distribution of natural habitats has been shrinking day by day due to extensive collection. Genetic diversity identifcation will likely assist in conserving the diferent Glycyrrhiza species. A total of 127 simple sequence repeat (SSR) markers from Medicago truncatula Gaertn., Phaseolus vulgaris L., and Cicer arietinum L. were used and 26 of them were amplifed on Glycyrrhiza genomes. Te highest transferability rate (33%) was obtained from M. truncatula markers, while the highest genetic diversity values were obtained in P. vulgaris markers. Te markers BM153 and PV-ag004 from P. vulgaris had the largest polymorphism information content. Te amplifed primers were also used to identify genetic diversity among three Glycyrrhiza species. Te gene diversity values among Glycyrrhiza species appeared to be similar; however, donor species mostly had lower diversity values than those of Glycyrrhiza species. Additionally, the genetic analysis showed that G. favescens Boiss. subsp. favescens is more distantly related to species G. glabra L. var. glandulifera and G. echinata L. Te number of alleles was mostly higher than in the donor species, possibly proposing a multiallelic and/or polyploid structure in Glycyrrhiza species.
Licorice (Glycyrrhiza spp.) is an important medicinal plant and its distribution of natural habitats has been shrinking day by day due to extensive collection. Genetic diversity identifcation will likely assist in conserving the diferent Glycyrrhiza species. A total of 127 simple sequence repeat (SSR) markers from Medicago truncatula Gaertn., Phaseolus vulgaris L., and Cicer arietinum L. were used and 26 of them were amplifed on Glycyrrhiza genomes. Te highest transferability rate (33%) was obtained from M. truncatula markers, while the highest genetic diversity values were obtained in P. vulgaris markers. Te markers BM153 and PV-ag004 from P. vulgaris had the largest polymorphism information content. Te amplifed primers were also used to identify genetic diversity among three Glycyrrhiza species. Te gene diversity values among Glycyrrhiza species appeared to be similar; however, donor species mostly had lower diversity values than those of Glycyrrhiza species. Additionally, the genetic analysis showed that G. favescens Boiss. subsp. favescens is more distantly related to species G. glabra L. var. glandulifera and G. echinata L. Te number of alleles was mostly higher than in the donor species, possibly proposing a multiallelic and/or polyploid structure in Glycyrrhiza species.
Açıklama
Anahtar Kelimeler
Orman Mühendisliği
Kaynak
Turkish Journal of Agriculture and Forestry
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
38
Sayı
1
